Induction of uncoupling protein-1 and-3 in brown adipose tissue by kaki-tannin in type 2 diabetic NSY/Hos mice
Induction of uncoupling protein-1 and-3 in brown adipose tissue by kaki-tannin in type 2 diabetic NSY/Hos mice
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DOI：
10.1016/j.fct.2011.10.067
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发表时间：
2012-02-01
影响因子：
4.3
通讯作者：
Yokoyama, Shin-ichiro
中科院分区：
文献类型：
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作者：
Matsumoto, Kenji;Yokoyama, Shin-ichiro
柿单宁是从柿（Diospyros kaki 'Hachiya'）幼果中提取的一种高聚合单宁，具有结合胆汁酸的活性。为了验证柿单宁对2型糖尿病中脂质和葡萄糖代谢的影响，2型糖尿病NSY/Hos小鼠被喂食补充有1%（w/w）柿单宁的AIN 76改良的高脂肪饮食8周。柿单宁诱导粪便胆汁酸排泄增加2倍，并在预防血浆胆固醇、甘油三酯和胰岛素水平升高方面显着有效。柿单宁治疗也防止脂肪肝。为了确定这些作用的分子机制，对肝脏、棕色脂肪组织（BAT）和骨骼肌进行了基因表达分析。与胆固醇代谢相关的基因，包括3-羟基-3-甲基戊二酰辅酶A还原酶和固醇调节元件结合蛋白2，在柿单宁组的肝脏中增加。有趣的是，解偶联蛋白-1（UCP 1）基因和UCP 3基因在柿单宁组的BAT中显著增加，这也在蛋白质水平上得到证实。这些结果表明，诱导UCP 1和UCP 3在BAT的柿单宁治疗可能会影响能量代谢，从而有助于2型糖尿病NSY/Hos小鼠的有益效果。(C)2011爱思唯尔有限公司保留所有权利。
Kaki-tannin, a highly polymerized-tannin from the young fruits of persimmon (Diospyros kaki 'Hachiya'), has been shown to have bile acid-binding activity. To verify the effect of kaki-tannin on the metabolism of lipid and glucose in type 2 diabetes, type 2 diabetic NSY/Hos mice were fed an AIN76-modified high fat diet supplemented with 1% (w/w) kaki-tannin for 8 weeks. Kaki-tannin induced a 2-fold increase in fecal bile acid excretion and was significantly effective in the prevention of a rise in plasma cholesterol, triglyceride, and insulin levels. Kaki-tannin treatment also prevented fatty liver. To identify the molecular mechanism underlying these effects, gene expression analysis was performed on liver, brown adipose tissue (BAT), and skeletal muscle. The genes related to cholesterol metabolism, including 3-hydroxy-3-methylglutaryl-coenzyme A reductase and sterol regulatory element-binding protein 2, were increased in the liver of the kaki-tannin group. Interestingly, the uncoupling protein-1 (UCP1) gene and the UCP3 gene were significantly increased in the BAT of the kaki-tannin group, which was also confirmed at the protein level. These findings indicated that induction of UCP1 and UCP3 in the BAT by kaki-tannin treatment might influence the energy metabolism, thus contributing beneficial effects to type 2 diabetic NSY/Hos mice. (C) 2011 Elsevier Ltd. All rights reserved.