Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor
Resistance to diamide insecticides in diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor
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DOI：
10.1016/j.ibmb.2012.09.001
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发表时间：
2012-11-01
影响因子：
3.8
通讯作者：
Nauen, Ralf
中科院分区：
文献类型：
--
作者：
Troczka, Bartek;Zimmer, Christoph T.;Nauen, Ralf
双酰胺杀虫剂如氯虫苯甲酰胺和氟虫双酰胺是一类选择性靶向昆虫兰尼碱受体（RyR）的新型杀虫剂，RyR是一类独特的同源四聚体钙释放通道，其在许多细胞类型中的钙稳态中起关键作用。最近有报道说，小菜蛾对这些杀虫剂产生了抗药性。小菜蛾（Plutella xylostella）是十字花科作物的全球性鳞翅目害虫。在本研究中，一个区域的基因编码的RyR从菲律宾和泰国收集的，并发现是超过200倍的氯虫苯甲酰胺和氟虫双酰胺的敏感菌株相比，敏感菌株的建议的二酰胺结合位点，通过RT-PCR扩增和测序。与几个敏感的参考菌株的序列的比较显示在每个耐药菌株中的非同义突变，在这两种情况下，导致蛋白质中的甘氨酸到谷氨酸的取代（G4946 E）。在两个地理上分离的小菜蛾抗性菌株中，在所提出的二酰胺结合位点的高度保守区域内的相同氨基酸取代的独立进化强烈表明与二酰胺抗性的因果关系。此外，我们设计了一种基于焦磷酸测序的诊断方法，用于抗性监测目的，可用于检测田间采集的小菜蛾样品中的G4946 E突变。报告的耐药性管理策略的研究结果的影响进行了讨论。(C)2012爱思唯尔有限公司保留所有权利。
Diamide insecticides such as chlorantraniliprole and flubendiamide are a new class of insecticide that selectively target insect ryanodine receptors (RyR), a distinct class of homo-tetrameric calcium release channels which play a pivotal role in calcium homeostasis in numerous cell types. Resistance to these insecticides has recently been reported in the diamondback moth. Plutella xylostella (Lepidoptera: Plutellidae), a global lepidopteran pest of cruciferous crops. In the present study a region of the gene encoding the proposed diamide binding site of the RyR from P. xylostella collected from the Philippines and Thailand and found to be over 200-fold resistant to both chlorantraniliprole and flubendiamide compared to susceptible strains, were amplified by RT-PCR and sequenced. Comparison of the sequence with those from several susceptible reference strains revealed non-synonymous mutations in each of the resistant strains that in both cases lead to a glycine to glutamic acid substitution (G4946E) in the protein. The independent evolution of the same amino acid substitution within a highly conserved region of the proposed diamide binding site in two geographically separated resistant strains of P. xylostella strongly suggests a causal association with diamide resistance. Furthermore we designed a pyrosequencing-based diagnostic assay for resistance monitoring purposes that can be used to detect the G4946E mutation in field-collected samples of diamondback moth. The implications of the reported findings for resistance management strategies are discussed. (C) 2012 Elsevier Ltd. All rights reserved.