A METHOD FOR RAPID MEASUREMENT OF HEAT OR COLD RESISTANCE OF SMALL INSECTS
A METHOD FOR RAPID MEASUREMENT OF HEAT OR COLD RESISTANCE OF SMALL INSECTS
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DOI：
10.2307/2389288
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发表时间：
1992-01-01
影响因子：
5.2
通讯作者：
WEBER, KE
中科院分区：
文献类型：
--
作者：
HUEY, RB;CRILL, WD;WEBER, KE
1.我们描述了一种快速测量许多小昆虫的耐热性或耐寒性的新技术。我们在一个温控柱中加热（或冷却）昆虫，并测量它们被击倒的温度。耐热性的人工选择实验表明，击倒温度对选择反应迅速。仅经过四代的选择，试验和对照品系在击倒温度和生理耐热性方面都存在显著差异，以热休克存活率为衡量标准。潜在的应用（驯化，老化，选择）和技术问题（身体大小，湿度，统计考虑）进行了评估。
1. We describe a new technique for rapidly measuring the heat or cold resistance of many small insects. We heat (or cool) insects in a temperature-controlled column and measure the temperature at which they are knocked down.2. An artificial selection experiment on heat resistance demonstrates that knock-down temperatures respond rapidly to selection. After only four generations of selection, the experimental and control lines differed significantly in both knock-down temperature and physiological heat tolerance, as measured by per cent survival of a heat shock.3. Potential applications (acclimation, ageing, selection) and technical problems (body size, humidity, statistical considerations) are evaluated.