Influence of titania matrix on retention behaviour in reversed-phase liquid chromatography
Influence of titania matrix on retention behaviour in reversed-phase liquid chromatography
复制标题
二氧化钛基质对反相液相色谱保留行为的影响
DOI：
10.1016/0021-9673(95)00456-4
复制
发表时间：
1996
影响因子：
4.1
通讯作者：
Yoshihito Suzuki
中科院分区：
文献类型：
--
作者：
K. Tani;Yoshihito Suzuki
采用溶胶-凝胶法在实验室规模上合成了二氧化钛。通过与十八烷基三乙氧基硅烷反应制备了钛基反相填料。在正相和反相条件下测试得到的二氧化钛和十八烷基改性的二氧化钛，以评估二氧化钛基质对保留的影响。在正相色谱中，二氧化钛显示出碱性性质，并强烈保留酸性分析物。酸性分析物的k′值随着表面覆盖度的增加而降低，因为十八烷基改性大大降低了二氧化钛基质对保留的影响。在反相色谱中，使用含有乙酸-乙酸钠缓冲液的移动的相，二氧化钛表现为阴离子交换剂，并且由于十八烷基修饰减少了阴离子交换位点的数量，因此二氧化钛基质对保留的影响减少。另一方面，虽然保留了苄胺，但未检测到二氧化钛的阳离子交换性质。十八烷基改性通过形成硅醇和十八烷基基团来增加苄胺的保留。
Titania was synthesized on a laboratory scale by the sol-gel method. Titania-based reversed-phase packings were prepared by reaction with octadecyltriethoxysilane. The titania and octadecyl-modified titanias obtained were tested under normal- and reversed-phase conditions to assess the influence of the titania matrix on retention. In normal-phase chromatography, the titania showed basic properties and strongly retained an acidic analyte. The k′ value of the acidic analyte decreased with increasing surface coverage because the influence of the titania matrix on retention was greatly reduced by octadecyl modification. In reversed-phase chromatography with the use of a mobile phase containing acetic acid-sodium acetate buffer, the titania behaved as an anion exchanger, and the influence of the titania matrix on retention was reduced because octadecyl modification decreased the number of anion-exchange sites. On the other hand, cation-exchange properties of titania were not detectable although benzylamine was retained. Octadecyl modification increased the retention of benzylamine by the formation of silanol and octadecyl groups.