Pluripotent stem cell-derived organoids: using principles of developmental biology to grow human tissues in a dish
Pluripotent stem cell-derived organoids: using principles of developmental biology to grow human tissues in a dish
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DOI：
10.1242/dev.140731
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发表时间：
2017-03-15
期刊：
影响因子：
4.6
通讯作者：
Wells, James M.
中科院分区：
文献类型：
--
作者：
McCauley, Heather A.;Wells, James M.
多能干细胞（PSC）衍生的类器官是通过在体外将发育生物学原理应用于PSC而产生的微型三维人体组织。产生类器官的方法使用定向分化、形态发生过程和内在驱动的细胞自组装的组合，所述细胞自组装模拟发育中胚胎中的器官发生。所得的类器官具有显著的细胞类型复杂性，结构和功能与其体内对应物相似。在过去的五年中，已经产生了具有所有三个胚层成分的人类PSC衍生的类器官，从而建立了一个新的人类模型系统。在这里，在随附的海报中，我们概述了发育生物学的原理如何在体外生成人类类器官，以及类器官现在如何被用作研究人类发育生物学的主要研究工具。
Pluripotent stem cell (PSC)-derived organoids are miniature, three-dimensional human tissues generated by the application of developmental biological principles to PSCs in vitro. The approach to generate organoids uses a combination of directed differentiation, morphogenetic processes, and the intrinsically driven self-assembly of cells that mimics organogenesis in the developing embryo. The resulting organoids have remarkable cell type complexity, architecture and function similar to their in vivo counterparts. In the past five years, human PSC-derived organoids with components of all three germ layers have been generated, resulting in the establishment of a new human model system. Here, and in the accompanying poster, we provide an overview of how principles of developmental biology have been essential for generating human organoids in vitro, and how organoids are now being used as a primary research tool to investigate human developmental biology.