Expression of Ets-related transcription factors and matrix metalloproteinase genes in human breast cancer cells
Expression of Ets-related transcription factors and matrix metalloproteinase genes in human breast cancer cells
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DOI：
10.1023/a:1014993006190
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发表时间：
2002-04-01
影响因子：
3.8
通讯作者：
Tozer, RG
中科院分区：
文献类型：
--
作者：
Barrett, JM;Puglia, MA;Tozer, RG
研究了两种不同侵袭潜能的乳腺癌细胞系中ets和MMP基因的表达水平。侵袭性越强的MDA-MB-231细胞系的Ets-1、Ets-2、PEA3、ERM、Tel、Net、MMP-13和-14 mRNA水平高于MCF-7细胞。MMP-1、-3和-16 mrna表达量相等。TPA刺激两种细胞系中MMP-1、-9和TIMP-1 mRNA的表达。两种细胞系均未检测到MMP-2和MMP-7 mrna。Ets-1蛋白仅在MDA-MB-231细胞中检测到，其水平在TPA刺激后升高。TPA诱导MCF-7细胞中MMP-9的活性，增加MDA-MB-231细胞中MMP-9的活性，但未检测到MMP-2的活性。
The expression levels of ets and MMP genes was examined in two breast cancer cell lines of differing invasive potential. The more invasive MDA-MB-231 cell line had higher levels of Ets-1, Ets-2, PEA3, ERM, Tel, Net, MMP-13 and -14 mRNA than MCF-7 cells. MMP-1, -3 and -16 mRNAs were expressed equally. TPA stimulated MMP-1, -9 and TIMP-1 mRNA expression in both cell lines. MMP-2 and MMP-7 mRNAs were not detected in either cell line. The Ets-1 protein was only detected in MDA-MB-231 cells and its level increased following TPA stimulation. TPA induced MMP-9 activity in MCF-7 cells and increased its activity in MDA-MB-231 cells, however, MMP-2 activity was not detected.