Direct and Indirect Control of Late Sporulation Genes by GerR of Bacillus subtilis
Direct and Indirect Control of Late Sporulation Genes by GerR of Bacillus subtilis
复制标题
DOI：
10.1128/jb.00329-10
复制
发表时间：
2010-07-01
影响因子：
3.2
通讯作者：
Ricca, Ezio
中科院分区：
文献类型：
--
作者：
Cangiano, Giuseppina;Mazzone, Antonio;Ricca, Ezio
GerR是枯草芽孢杆菌的孢子形成特异性转录因子，其已被鉴定为由含sigma（E）的RNA聚合酶转录的基因的负调节因子和作为三个晚期孢子形成基因表达的正效应因子。在这里，我们证实gerR转录依赖于含有sigma（E）的RNA聚合酶，但也观察到它需要转录调节因子SpoIIID。GerR在调节几个晚期孢子形成基因表达中的作用的研究使我们能够观察到其对spoVIF、cotC和cotG的作用是强阳性的，对cotB的作用是弱阳性的，对cotU的作用是阴性的。染色质免疫沉淀（ChIP）实验结果表明，GerR与部分（但不是全部）GerR控制基因的启动子区结合，由此我们提出GerR通过两种方式控制晚期孢子形成基因：（i）直接作用于cotB、cotU和spoVIF的转录;和（ii）通过SpoVIF的激活间接地，SpoVIF稳定转录激活因子GerE并因此诱导依赖于GerE的基因cotC和cotG的表达。
GerR is a sporulation-specific transcriptional factor of Bacillus subtilis that has been identified as a negative regulator of genes transcribed by sigma(E)-containing RNA polymerase and as a positive effector of the expression of three late sporulation genes. Here we confirmed that gerR transcription is dependent on sigma(E)-containing RNA polymerase but also observed that it requires the transcriptional regulator SpoIIID. The study of the role of GerR in regulating the expression of several late sporulation genes allowed us to observe that its effect is strongly positive on spoVIF, cotC, and cotG, weakly positive on cotB, and negative on cotU. The results of chromatin immunoprecipitation (ChIP) experiments indicated that GerR binds to the promoter regions of some, but not all, of the GerR-controlled genes, leading us to propose that GerR controls late sporulation genes in two ways: (i) directly, by acting on the transcription of cotB, cotU and spoVIF; and (ii) indirectly, through the activation of SpoVIF, which stabilizes the transcriptional activator GerE and consequently induces the expression of the GerE-dependent genes cotC and cotG.