Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA
Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA
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DOI：
10.1111/j.1750-3841.2012.02756.x
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发表时间：
2012-07-01
影响因子：
3.9
通讯作者：
Stankovic, Miroslava
中科院分区：
文献类型：
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作者：
Godevac, Dejan;Tesevic, Vele;Stankovic, Miroslava
根据 LC/UV/MS 分析，从黑醋栗、红醋栗和白醋栗（各 2 个品种）种子提取物中鉴定出 35 种化合物。黑醋栗品种含有原儿茶酸和对羟基苯甲酸，以及微量的含有酚酸的腈。突触酰基葡萄糖苷的存在是 cv 的特征。马尔林宝石。倍半萜苷和羧甲基吲哚苷主要存在于白醋栗和红醋栗品种中。黑加仑种子含有较高含量的黄酮类化合物，尤其是芦丁、异槲皮素和花旗松素。使用细胞松弛素-B 封闭微核 (CBMN) 测定法测试了醋栗种子提取物对人外周淋巴细胞染色体畸变的体外保护作用。对双核细胞中的 MN 频率进行评分，黑加仑品种表现出最佳的抗氧化潜力。种子提取物的浓度仅为 0.17 mg/mL。与对照细胞培养物相比，Malling Jewel 使 MN 频率降低了 60%。实际应用：我们的结果提供了醋栗种子提取物和分离的纯化合物对人类淋巴细胞细胞遗传学损伤的保护作用的证据。因此，醋栗种子提取物可以对多种疾病发挥有益作用，因为许多生物作用归因于其抗氧化特性。
On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures. Practical Application: Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.