Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
复制标题
DOI：
10.1016/j.jmb.2015.06.014
复制
发表时间：
2015-11-20
影响因子：
5.6
通讯作者：
Kovács ÁT
中科院分区：
文献类型：
--
作者：
Hölscher T;Bartels B;Lin YC;Gallegos-Monterrosa R;Price-Whelan A;Kolter R;Dietrich LEP;Kovács ÁT
生物膜的形成是一个复杂的过程，涉及多种信号通路和基因表达的变化。许多的感觉机制和监管级联已被定义为生物膜所形成的各种生物附着在固体表面。相比之下，我们对气液界面生物膜（即薄膜）形成的基本机制的了解要少得多。特别是，鞭毛的作用已经在多个固体表面生物膜模型中进行了研究，但在很大程度上仍未确定的薄膜。在这项工作中，我们的特点的贡献，鞭毛为基础的运动，趋化性和氧传感器在革兰氏阳性枯草芽孢杆菌的表膜形成。我们证实，鞭毛为基础的运动参与，但不是绝对必要的，B。枯草菌表膜形成。此外，我们表明，鞭毛为基础的运动性，趋化性和氧传感是重要的成功竞争期间B。枯草菌表膜形成。我们报告说，鞭毛为基础的运动同样有助于在革兰氏阴性铜绿假单胞菌的竞争试验中的薄膜形成和健身。静态液体培养的延时成像显示，在B。在枯草杆菌和铜绿假单胞菌中，在管中形成湍流，并且就在形成表膜之前在气-液界面下方出现透明区，但仅在具有鞭毛的菌株中。
Biofilm formation is a complex process involving various signaling pathways and changes in gene expression. Many of the sensory mechanisms and regulatory cascades involved have been defined for biofilms formed by diverse organisms attached to solid surfaces. By comparison, our knowledge of the basic mechanisms underlying the formation of biofilms at air-liquid interfaces, i.e. pellicles, is much less complete. In particular, the roles of flagella have been studied in multiple solid-surface biofilm models, but remain largely undefined for pellicles. In this work, we characterize the contributions of flagellum-based motility, chemotaxis and oxygen sensing to pellicle formation in the Gram-positive Bacillus subtilis. We confirm that flagellum-based motility is involved in, but is not absolutely essential for, B. subtilis pellicle formation. Further, we show that flagellum-based motility, chemotaxis, and oxygen sensing are important for successful competition during B. subtilis pellicle formation. We report that flagellum-based motility similarly contributes to pellicle formation and fitness in competition assays in the Gram-negative Pseudomonas aeruginosa. Time-lapse imaging of static liquid cultures demonstrates that in both B. subtilis and P. aeruginosa, a turbulent flow forms in the tube and a zone of clearing appears below the air-liquid interface just before the formation of the pellicle, but only in strains that have flagella.