Purification and characterization of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine, a novel antibacterial substance of sarcophaga peregrina (flesh fly)
Purification and characterization of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine, a novel antibacterial substance of sarcophaga peregrina (flesh fly)
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DOI：
10.1074/jbc.271.23.13573
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发表时间：
1996-06-07
影响因子：
4.8
通讯作者：
Natori, S
中科院分区：
文献类型：
--
作者：
Leem, JY;Nishimura, C;Natori, S
We purified a novel antibacterial substance from immunized adult Sarcophaga and determined its molecular structure to be N-beta-alanyl-5-S-glutathionyl 3,4-dihydroxyphenylalanine (5-S-GAD), We synthesized 5-S-GAD enzymatically from N-beta-alanyl-3,4-dihydroxyphenylalanine (beta-Ala-Dopa) and reduced glutathione (GSH), The antibacterial activity of 5-S-GAD was found to be due to its production of H2O2. This is a novel antibacterial mechanism as it differs from the mechanisms of known antibacterial peptides, Two possible roles of 5-S-GAD in insect immunity, suppression of bacterial growth and activation of a Rel family transcription factor, are proposed.
