SIMULTANEOUS EXPRESSION OF 2 DIFFERENT TYPES OF INTERMEDIATE SIZED FILAMENTS IN MOUSE KERATINOCYTES PROLIFERATING INVITRO
SIMULTANEOUS EXPRESSION OF 2 DIFFERENT TYPES OF INTERMEDIATE SIZED FILAMENTS IN MOUSE KERATINOCYTES PROLIFERATING INVITRO
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DOI：
10.1111/j.1432-0436.1979.tb01010.x
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发表时间：
1979-01-01
期刊：
影响因子：
2.9
通讯作者：
WEBER, K
中科院分区：
文献类型：
--
作者：
FRANKE, WW;SCHMID, E;WEBER, K
通过免疫荧光显微镜检查源自小鼠皮肤的表皮角质形成细胞和源自体外生长的小鼠角质形成细胞自发转化的已建立细胞系（HEL [小鼠表皮C3H细胞]）中存在的中等大小的细丝。这是通过使用针对不同类别的中等大小的丝的亚基蛋白的抗体，并通过EM和凝胶电泳的细胞骨架制剂高度富集在中等大小的丝。源自新生儿皮肤的角质形成细胞在体外仅能够有限复制，仅显示单一类型的中等大小的细丝，即，含有前角蛋白的张力原纤维样细丝阵列。在体外无限增殖的HEL细胞保留分化的表皮细胞典型的张力调节样结构，并显示波形蛋白型的中等大小的细丝，即，间充质和间充质衍生细胞的典型细丝系统。培养的表皮细胞中的波形蛋白型细丝可能与转化状态或体外生长条件有关，并且在体外增殖的其他分化的上皮细胞可能具有多于一个的中等大小细丝系统。
The intermediate-sized filaments present in epidermal keratinocytes derived from mouse skin and in an established cell line (HEL [murine epidermal C3H cells]) derived from spontaneous transformation of murine keratinocytes grown in vitro, were examined by immunofluorescence microscopy. This was done by using antibodies directed against subunit proteins of different classes of intermediate-sized filaments, and by EM and gel electrophoresis of cytoskeletal preparations highly enriched in intermediate-sized filaments. The keratinocytes derived from neonatal skin, which are capable of only limited replication in vitro, show only a single type of intermediate-sized filaments, i.e., the tonofibril-like arrays of filaments containing prekeratin. HEL cells, which proliferate indefinitely in vitro, retain the tonofilament-like structures typical of differentiated epidermal cells and display intermediate-sized filaments of the vimentin type, i.e., the filament system typical of mesenchymal and mesenchyme-derived cells. Vimentin-type filaments in epidermal cells in culture may be related to the transformed state or in vitro growth conditions and other differentiated epithelial cells proliferating in vitro may have more than 1 system of intermediate-sized filaments.