CD4+ T-cells are important in regulating macrophage polarization in C57BL/6 wild-type mice
CD4+ T-cells are important in regulating macrophage polarization in C57BL/6 wild-type mice
复制标题
DOI：
10.1016/j.cellimm.2010.10.002
复制
发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Ashkar, Ali A.
中科院分区：
文献类型：
--
作者：
Chan, Tiffany;Pek, Elisabeth A.;Ashkar, Ali A.
在活化过程中，巨噬细胞发生影响其表面蛋白表达和细胞因子产生的生理变化，并随后被分为M1（经典活化）和M2（交替活化）巨噬细胞。目前尚不清楚是哪种淋巴细胞群提供了调节巨噬细胞极化的免疫微环境。在这项研究中，我们建立了C57BL/6野生型小鼠腹腔巨噬细胞的功能和表型谱。我们还发现Rag1（-/-）和Rag2(-/-) γ c（-/-）小鼠与对照小鼠相比具有相似的、夸张的M1特征，这表明NK和/或NK- t细胞在这一过程中可能不是必需的。通过控制环境因素，我们确定淋巴细胞来源的细胞因子，而不是巨噬细胞本身的固有特性，对巨噬细胞的调节至关重要。最后，我们报告了来自CD4（-/-）小鼠的巨噬细胞显示M1谱，这表明CD4(+) t细胞在提供细胞因子环境以调节巨噬细胞向正常野生型条件下的M2谱方面比其他淋巴细胞群发挥主导作用。(c) 2010爱思唯尔公司版权所有。
During activation, macrophages undergo physiological changes affecting their surface protein expression and cytokine production and have been subsequently categorized into M1 (classically-activated) and M2 (alternatively-activated) macrophages. It remains unclear which lymphocyte population provides the immune microenvironment to regulate macrophage polarization. In this study, we establish a functional and phenotypic profile of peritoneal macrophages from C57BL/6 wild-type mice. We also showed that Rag1(-/-) and Rag2(-/-)gamma c(-/-) mice have similar, exaggerated M1 characteristics in comparison to control mice, suggesting that NK and/or NK-T cells may not be essential in this process. By controlling for environmental factors, we determine that lymphocyte-derived cytokines, rather than inherent properties of macrophages themselves, are crucial for their regulation. Lastly, we report that macrophages from CD4(-/-) mice display an M1 profile, suggesting that CD4(+) T-cells play a dominant role over other lymphocyte populations in providing the cytokine environment for regulating macrophages towards an M2 profile under normal wild-type conditions. (c) 2010 Elsevier Inc. All rights reserved.