CHARACTERIZATION OF THE F-PLASMID BIFUNCTIONAL CONJUGATION GENE, TRAG
CHARACTERIZATION OF THE F-PLASMID BIFUNCTIONAL CONJUGATION GENE, TRAG
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DOI：
10.1007/bf00299147
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发表时间：
1992-03-01
期刊：
影响因子：
--
通讯作者：
SKURRAY, R
中科院分区：
文献类型：
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作者：
FIRTH, N;SKURRAY, R
在接合过程的两个阶段：菌毛的生物合成和交配聚集体的稳定，都需要大肠杆菌F质粒基因TRAG。测定了TRAG的核苷酸序列，并通过酶可及性实验分析了其产物在细胞膜中的拓扑结构。利用质粒缺失进行的互补分析表明，蛋白质产物的N端周质片段(TraGp)与其菌毛组装活性之间存在相关性。抗TraGp抗血清的制备促进了TraGp*的检测，TraGp*可能是TraGp的一种内部切割产物。虽然它的功能尚不清楚，但TraGp*位于周质中，并已被证明具有聚集稳定所需的序列。TraGp*的检测增加了TRAG的两种功能由不同产品执行的可能性。
The Escherichia coli F plasmid gene, traG, is required for two stages of the conjugation process: pilus biosynthesis and mating aggregate stabilization. The nucleotide sequence of traG has been determined and the topology of its product in the cytoplasmic membrane analysed using protease accessibility experiments. Complementation analysis employing plasmid deletions revealed a correlation between an N-terminal periplasmic segment of the protein product (TraGp) and its pilus assembly activity. Production of an anti-TraGp antiserum has facilitated the detection of TraGp*, a possible internal cleavage product of TraGp. Although its function is unknown, TraGp* is located in the periplasm and has been shown to possess sequences required for aggregate stabilization. The detection of TraGp* raises the possibility that the two functions of traG are carried out by separate products.