Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand, troglitazone, on colon cancer cells
Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARγ ligand, troglitazone, on colon cancer cells
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DOI：
10.1016/j.bbagen.2004.08.012
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发表时间：
2004-11-18
影响因子：
3
通讯作者：
Miyashita, K
中科院分区：
文献类型：
--
作者：
Hosokawa, M;Kudo, M;Miyashita, K
研究了从食用海藻裙带菜中提取的岩藻黄质对结肠癌细胞存活率和诱导细胞凋亡的影响。岩藻黄质显著降低人结肠癌细胞系Caco-2、HT-29和DLD-1的存活率。此外，岩藻黄质处理会导致DNA断裂，表明细胞发生了凋亡。22.6µM岩藻黄质作用24 h后，Caco-2细胞DNA片段化程度为对照组的10倍。岩藻黄质可抑制Bcl2蛋白的表达。Caspase抑制剂Z-VAD-fmk可部分抑制岩藻黄质诱导的DNA断裂。此外，3.8um岩藻黄质和10um曲格列酮(PPAR-激活型受体(PPAR)的特异性配体)联合作用可有效降低Caco-2细胞的存活率。然而，用相同浓度的岩藻黄质和曲格列酮分开处理并不影响细胞存活率。这些发现表明，岩藻黄质可能通过与曲格列酮联合调节细胞活力而作为结肠癌细胞的化学预防和/或化疗类胡萝卜素。(C)2004爱思唯尔B.V.保留所有权利。
The effect of fucoxanthin, from the edible seaweed Undaria pinnatifida on viability of colon cancer cells and induction of apoptosis was investigated. Fucoxanthin remarkably reduced the viability of human colon cancer cell lines, Caco-2, HT-29 and DLD-1. Furthermore, treatment with fucoxanthin induced DNA fragmentation, indicating apoptosis. The DNA fragmentation in Caco-2 cells treated with 22.6 muM fucoxanthin for 24 h was 10-fold higher than in the control. Fucoxanthin suppressed the level of Bcl-2 protein. Also, DNA fragmentation induced by fucoxanthin was partially inhibited by a caspase inhibitor Z-VAD-fmk. Moreover, combined treatment with 3.8 muM fucoxanthin and 10 muM troglitazone, which is a specific ligand for peroxisome proliferator-activated receptor (PPAR)gamma, effectively decreased the viability of Caco-2 cells. However, separate treatments with these same concentrations of fucoxanthin nor troglitazone did not affect cell viability. These findings indicate that fucoxanthin may act as a chemopreventive and/or chemotherapeutic carotenoid in colon cancer cells by modulating cell viability in combination with troglitazone. (C) 2004 Elsevier B.V. All rights reserved.