Selective Killing of Pathogenic Bacteria by Antimicrobial Silver Nanoparticle-Cell Wall Binding Domain Conjugates
Selective Killing of Pathogenic Bacteria by Antimicrobial Silver Nanoparticle-Cell Wall Binding Domain Conjugates
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DOI：
10.1021/acsami.8b00181
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发表时间：
2018-04-25
影响因子：
9.5
通讯作者：
Dordick, Jonathan S.
中科院分区：
文献类型：
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作者：
Kim, Domyoung;Kwon, Seok-Joon;Dordick, Jonathan S.
广谱抗生素不加区别地杀死细菌，消除非病原微生物并导致抗生素耐药菌株的进化。可以选择性杀死致病菌而不针对自然微生物群落或微生物组中的其他细菌的特定抗菌药物可能能够解决这一问题。在这项工作中，我们证明，适当地与选择性细胞壁结合域（CBD）缀合的银纳米颗粒可以有效地靶向并选择性地杀死细菌。作为一个相关的例子，来自炭疽芽孢杆菌的 CBDBA 在与枯草芽孢杆菌的混合物以及与金黄色葡萄球菌的混合物中选择性地结合炭疽芽孢杆菌。因此，这种新的生物辅助杂交策略有可能提供病原菌的选择性净化，同时对正常微生物群的影响最小。
Broad-spectrum antibiotics indiscriminately kill bacteria, removing nonpathogenic microorganisms and leading to evolution of antibiotic resistant strains. Specific antimicrobials that could selectively kill pathogenic bacteria without targeting other bacteria in the natural microbial community or microbiome may be able to address this concern. In this work, we demonstrate that silver nanoparticles, suitably conjugated to a selective cell wall binding domain (CBD), can efficiently target and selectively kill bacteria. As a relevant example, CBDBA from Bacillus anthracis selectively bound to B. anthracis in a mixture with Bacillus subtilis, as well in a mixture with Staphylococcus aureus. This new biologically-assisted hybrid strategy, therefore, has the potential to provide selective decontamination of pathogenic bacteria with minimal impact on normal microflora.