High concentrate diet induced mucosal injuries by enhancing epithelial apoptosis and inflammatory response in the hindgut of goats.
High concentrate diet induced mucosal injuries by enhancing epithelial apoptosis and inflammatory response in the hindgut of goats.
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高浓度饮食通过增强山羊后肠上皮细胞凋亡和炎症反应而引起粘膜损伤
DOI：
10.1371/journal.pone.0111596
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhao R
中科院分区：
文献类型：
--
作者：
Tao S;Duanmu Y;Dong H;Ni Y;Chen J;Shen X;Zhao R
目的 人们普遍认为，在饲喂含有大量谷物的饲料的反刍动物的消化道中，脂多糖和挥发性脂肪酸 (VFA) 会积聚。与瘤胃上皮相比，后肠上皮由单层结构组成，脂多糖更“泄漏”，并且容易受到有机酸诱导的损伤。本研究的目的是调查饲喂高浓度饲料 6 周的山羊后肠上皮结构、细胞凋亡和炎症反应的变化。实验设计将8只带有瘤胃插管的本地山羊随机分为两组：一组饲喂高浓度饲料（65％浓缩干物质，HC），另一组饲喂低浓缩饲料（35％浓缩干物质，LC），连续6周。收集瘤胃液、血浆和后肠粘膜组织。采用组织学技术、实时PCR和蛋白质印迹来评估后肠的组织结构、细胞凋亡和局部炎症。结果饲喂HC日粮6周后，HC山羊瘤胃pH值显着降低（p<0.01），瘤胃脂多糖浓度显着升高（p<0.05）。在 HC 山羊中观察到后肠粘膜上皮和细胞间紧密连接的明显损伤，但在 LC 山羊中没有。与LC相比，HC山羊结肠上皮中MyD88和caspase-8 mRNA的表达增加（p<0.05），并且TLR-4和caspase-3的表达呈增加趋势。在 HC 山羊盲肠中，IL-1β mRNA 表达下降（p<0.05），而 caspase-3 表达可能增加（p = 0.07）。 HC 山羊结肠上皮中 NF-κB 蛋白水平升高。 Caspase-3 活性在结肠和盲肠中均升高，而 caspase-8 活性仅在结肠中统计学上升高。结论 饲喂高精饲料6周，通过激活上皮细胞凋亡和局部炎症反应，导致山羊后肠黏膜损伤。
Purpose It is widely accepted that lipopolysaccharide and volatile fatty acids (VFA) accumulate in the digestive tract of ruminants fed diets containing high portions of grain. Compared to the ruminal epithelium, the hindgut epithelium is composed of a monolayer structure that is more “leaky” for lipopolysaccharide and susceptible to organic acid-induced damage. The aim of this study was to investigate changes in epithelial structure, apoptosis and inflammatory response in the hindgut of goats fed a high-concentrate diet for 6 weeks. Experimental Design Eight local Chinese goats with rumen cannulas were randomly assigned to two groups: one group was fed a high-concentrate diet (65% concentrate of dry matter, HC) and the other group was fed a low-concentrate diet (35% concentrate of dry matter, LC) for 6 wks. Ruminal fluid, plasma, and hindgut mucosa tissues were collected. Histological techniques, real-time PCR and western blotting were used to evaluate the tissues structure, cell apoptosis and local inflammation in the hindguts. Results Feeding HC diet for 6 wks resulted in a significant decrease of ruminal pH (p<0.01), and ruminal lipopolysaccharide concentrations were significantly increased in HC goats (p<0.05). Obvious damage was observed to mucosal epithelium of the hindgut and the intercellular tight junctions in HC, but not in LC, goats. The expression of MyD88 and caspase-8 mRNA was increased in colonic epithelium of HC goats compared to LC (p<0.05), and the expression of TLR-4 and caspase-3 showed a tendency to increase. In the cecum, interleukin-1β mRNA expression was decreased (p<0.05), and caspase-3 showed a potential increase (p = 0.07) in HC goats. The level of NF-κB protein was increased in colonic epithelium of HC goats. Caspase-3 activity was elevated in both colon and cecum, whereas caspase-8 activity was statistically increased only in colon. Conclusions Feeding a high-concentrate diet to goats for 6 wks led to hindgut mucosal injuries via activating epithelial cells apoptosis and local inflammatory response.
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DOI：
10.1113/expphysiol.1989.sp003237
发表时间：
1989-01-01
影响因子：
--
作者：
GAEBEL, G;BELL, M;MARTENS, H
通讯作者：
MARTENS, H
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Madsen, K. L.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.
影响因子：
3.3
作者：
Metzler-Zebeli, B. U.;Hollmann, M.;Zebeli, Q.
通讯作者：
Zebeli, Q.
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Dunn, S. M.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.
山羊瘤胃上皮的细胞保护因子的下调喂养了高能量饮食。
影响因子：
3.7
作者：
Hollmann M;Miller I;Hummel K;Sabitzer S;Metzler-Zebeli BU;Razzazi-Fazeli E;Zebeli Q
通讯作者：
Zebeli Q