The redox state regulates RNA degradation in the chloroplast of Chlamydomonas reinhardtii.
The redox state regulates RNA degradation in the chloroplast of Chlamydomonas reinhardtii.
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氧化还原状态调节莱茵衣藻叶绿体中的 RNA 降解。
DOI：
10.1104/pp.121.4.1367
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发表时间：
1999
期刊：
影响因子：
7.4
通讯作者：
U. Klein
中科院分区：
文献类型：
--
作者：
M. Salvador;U. Klein
一种莱茵衣藻叶绿体DNA，命名为MU 7，携带嵌合的（rbcL启动子：β-葡糖醛酸糖苷酶[GUS]：psaB 3'端）基因，其转录本先前已被发现在光照下不稳定（在光照下半衰期为20分钟，而在黑暗中半衰期为5小时），研究了叶绿体转录本在光照下降解过程中电子传递和氧化还原状态的作用。用3-（3，4-二氯苯基）-1，1-二甲基脲（DCMU）阻断光合电子传递可防止MU 7细胞中GUS转录本库的光依赖性破坏。二酰胺，氧化剂，引起了可测量的延迟在光中的GUS转录物的降解。将二硫苏糖醇（DTT）（一种二硫醇还原剂）添加到MU 7细胞中，其中GUS转录物水平通过DCMU诱导的GUS转录物降解而稳定。类似地，DTT在光/暗循环的暗期（GUS转录物水平通常增加的时期）中加入MU 7细胞时诱导GUS转录物水平的降低。DCMU和DTT对内源叶绿体基因转录水平的影响与GUS转录水平的影响方向相同。这些结果表明，氧化还原状态在叶绿体转录本降解过程中起着调节作用。莱因哈德氏菌
A Chlamydomonas reinhardtii chloroplast transformant, designated MU7, carrying a chimeric (rbcL promoter: beta-glucuronidase [GUS]: psaB 3' end) gene whose transcripts have been found previously to be unstable in light (half-life of 20 min in light as opposed to a half-life of 5 h in the dark), was used to study the role of electron transport and of the redox state in the degradation of chloroplast transcripts in the light. Blocking photosynthetic electron transport with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) prevented the light-dependent breakdown of the pool of GUS transcripts in MU7 cells. Diamide, an oxidizing agent, caused a measurable delay in the degradation of GUS transcripts in the light. The addition of dithiothreitol (DTT), a dithiol reductant, to MU7 cells in which GUS transcript levels were stabilized by DCMU induced degradation of GUS transcripts. Similarly, DTT induced a decrease in the levels of GUS transcripts when added to MU7 cells in the dark period of the light/dark cycle, a period in which GUS transcript levels normally increase. The levels of transcripts of endogenous chloroplast genes were affected by DCMU and DTT in the same direction as levels of GUS transcripts. The results suggest a regulatory role of the redox state in the degradation of chloroplast transcripts in C. reinhardtii.
影响因子：
56.9
作者：
Kim, JM;Mayfield, SP
通讯作者：
Mayfield, SP
Poly(A) 结合蛋白在叶绿体中作为信息特异性翻译因子发挥作用。
DOI：
10.1073/pnas.95.5.2238
发表时间：
1998
影响因子：
11.1
作者：
Yohn,CB;Cohen,A;Danon,A;Mayfield,SP
通讯作者：
Mayfield,SP
影响因子：
56.9
作者：
BOYNTON, JE;GILLHAM, NW;SANFORD, JC
通讯作者：
SANFORD, JC