E-cigarettes: Effects in phagocytosis and cytokines response against Mycobacterium tuberculosis
E-cigarettes: Effects in phagocytosis and cytokines response against Mycobacterium tuberculosis
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DOI：
10.1371/journal.pone.0228919
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发表时间：
2020-02-10
期刊：
影响因子：
3.7
通讯作者：
Dominguez, Jose
中科院分区：
文献类型：
--
作者：
Gomez, Andromeda-Celeste;Rodriguez-Fernandez, Pablo;Dominguez, Jose
吸烟和肺结核是全球死亡的重要原因。一些流行病学研究表明，吸烟是结核病的一个危险因素。电子烟最近似乎是传统吸烟的一种更健康的替代品，尽管它们对结核病的影响尚不清楚。本研究旨在探讨电子烟对结核分枝杆菌吞噬功能和细胞因子产生的影响。通过将THP-1巨噬细胞暴露于四种电子蒸汽提取物进行体外感染，并测定M.结核病是确定的。通过共聚焦显微镜和Luminex的细胞因子产生来评估感染的百分比。细胞内M.在THP 1巨噬细胞暴露于电子蒸汽提取物后，发现了结核菌负荷;当使用牛分枝杆菌BCG-GFP菌株时，通过共聚焦显微镜观察到相同的趋势。电子香烟刺激促炎细胞因子反应。我们的结论是，电子香烟损害吞噬功能和细胞因子对M。结核
Cigarette smoking and tuberculosis are a significant cause of death worldwide. Several epidemiological studies have demonstrated cigarette smoking is a risk factor for tuberculosis. Electronic cigarettes have recently appeared as a healthier alternative to conventional smoking, although their impact in tuberculosis is not well understood. The aim of this study was to explore the effect of electronic cigarettes in phagocytosis of Mycobacterium tuberculosis and cytokines production. In vitro infection was carried out by exposing THP-1 macrophages to four electronic vapor extracts and the intracellular burden of M. tuberculosis was determined. The percentage of infection was evaluated by confocal microscopy and the cytokine production by Luminex. A reduction of intracellular M. tuberculosis burden in THP1 macrophages was found after its exposure to electronic vapor extract; the same trend was observed by confocal microscopy when Mycobacterium bovis BCG-GFP strain was used. Electronic cigarettes stimulate a pro-inflammatory cytokine response. We conclude that electronic cigarettes impair the phagocytic function and the cytokine response to M. tuberculosis.