Aquaporin-related proteins in the filter chamber of homopteran insects
Aquaporin-related proteins in the filter chamber of homopteran insects
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DOI：
10.1007/s004410050916
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发表时间：
1997-10-01
影响因子：
3.6
通讯作者：
Hubert, JF
中科院分区：
文献类型：
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作者：
LeCaherec, F;Guillam, MT;Hubert, JF
在同翅目昆虫中，植物木质部喂食器的消化道具有高度分化的部分，称为过滤室。在该组织中，水通过跨上皮渗透梯度穿过质膜。在之前对绿蝉过滤室的研究中，我们从质膜中纯化并表征了一种 25 kDa 的蛋白质，我们证明该蛋白质是水通道蛋白（或水通道，主要内在蛋白质家族的成员，一组小溶质的膜通道）。我们将这种蛋白质称为 AQPcic，即蝉水通道蛋白。在本研究中，我们使用抗 AQPcic 多克隆抗体对 Cercopis sanguinolenta、Philaenus spumarius、Aphrophora alni (Cercopidae)、Euscelidius variegatus 和 Scaphoideus titanus (Jassidae) 的肠道进行蛋白质印迹和免疫细胞化学分析。蛋白质印迹实验表明，在这些物种中发现了免疫相关的 AQPcic 蛋白。这些蛋白质的分子量为 15-26 kDa。对超薄过滤室切片的免疫细胞化学研究表明，抗 AQPcic 抗体系统地标记了上皮细胞的膜微绒毛。因此，这些细胞的生理学与其膜中水通道蛋白相关蛋白的存在之间存在良好的相关性。
In the Homopteran order of insects, the plant xylem feeders exhibit a highly differentiated part of their digestive tract known as the filter chamber. In this tissue, water crosses plasma membranes through a transepithelial osmotic gradient. In previous studies on the filter chamber of Cicadella viridis, we purified and characterized from the plasma membranes a 25 kDa protein that we demonstrated to be an aquaporin (or water channel, member of the major intrinsic protein family, a group of membrane channels for small solutes). We called this protein AQPcic for Cicadella aquaporin. In the present study, we used polyclonal antibody anti-AQPcic in Western blotting and immunocytochemical analysis of the intestinal tract of Cercopis sanguinolenta, Philaenus spumarius, Aphrophora alni (Cercopidae), Euscelidius variegatus, and Scaphoideus titanus (Jassidae). Western blotting experiments revealed that immunologically related AQPcic proteins are found in those species. The molecular weight of these proteins is 15-26 kDa. Immunocytochemical studies on ultrathin filter-chamber sections revealed that the anti-AQPcic antibody systematically labelled the membrane microvilli of epithelial cells. A good correlation thus exists between the physiology of these cells and the presence of aquaporin-related proteins in their membranes.