Noncoding RNAs of trithorax response elements recruit Drosophila Ash1 to ultrabithorax (Retracted Article)
Noncoding RNAs of trithorax response elements recruit Drosophila Ash1 to ultrabithorax (Retracted Article)
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DOI：
10.1126/science.1117705
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发表时间：
2006-02-24
期刊：
影响因子：
56.9
通讯作者：
Sauer, F
中科院分区：
文献类型：
--
作者：
Sanchez-Elsner, T;Gou, DW;Sauer, F
同源异型基因含有顺式调节三胸反应元件（TRES），其被表观遗传激活剂靶向并以组织特异性方式转录。我们发现，位于果蝇同源异型基因Ultrabithorax（UBX）的三个TREs的转录本通过招募表观遗传调节因子Ash1到模板TREs来介导转录激活。TRE转录与Ubx转录一致，并在果蝇中将Ash1募集到TRE。Ash1的SET结构域结合所有三种TRE转录物，每个TRE转录物仅与染色质中相应的TRE杂交并募集Ash1。TRE转录本的转基因转录恢复了Ash1向Ubx TRE的募集，并恢复了缺乏内源性TRE转录本的果蝇细胞和组织中Ubx的表达。小干扰RNA诱导的TRE转录物降解减弱了Ash1对TRES和Ubx表达的招募，这表明非编码TRE转录物在基因表达的表观遗传激活中起重要作用。
Homeotic genes contain cis-regulatory trithorax response elements (TREs) that are targeted by epigenetic activators and transcribed in a tissue-specific manner. We show that the transcripts of three TREs located in the Drosophila homeotic gene Ultrabithorax (Ubx) mediate transcription activation by recruiting the epigenetic regulator Ash1 to the template TREs. TRE transcription coincides with Ubx transcription and recruitment of Ash1 to TREs in Drosophila. The SET domain of Ash1 binds all three TRE transcripts, with each TRE transcript hybridizing with and recruiting Ash1 only to the corresponding TRE in chromatin. Transgenic transcription of TRE transcripts restores recruitment of Ash1 to Ubx TREs and restores Ubx expression in Drosophila cells and tissues that lack endogenous TRE transcripts. Small interfering RNA-induced degradation of TRE transcripts attenuates Ash1 recruitment to TREs and Ubx expression, which suggests that noncoding TRE transcripts play an important role in epigenetic activation of gene expression.