Molecular cloning and characterization of NPR1 gene from Arachis hypogaea
Molecular cloning and characterization of NPR1 gene from Arachis hypogaea
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DOI：
10.1007/s11033-014-3395-z
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发表时间：
2014-05
影响因子：
2.8
通讯作者：
Qi Wu;X. Wang;Y. Tang;H. Yu;Yu Ding;Chuanjian Yang;F. Cui;Jian Cheng Zhang;C. Wang
中科院分区：
文献类型：
--
作者：
Qi Wu;X. Wang;Y. Tang;H. Yu;Yu Ding;Chuanjian Yang;F. Cui;Jian Cheng Zhang;C. Wang
NPR1基因是植物抗病性的重要调节因子。采用快速cDNA末端扩增-聚合酶链反应(RACE-PCR)技术从花生品种日华1号中克隆了NPR1基因的cDNA。花生 NPR1 的全长 cDNA 由 2,078 个碱基对组成，开放阅读框长 1,446 bp，编码 481 个氨基酸。预测的 NPR1 包含高度保守的功能结构域（从 M1 到 D116 的 BTB/POZ 结构域）、蛋白质-蛋白质相互作用结构域（从 K158 到 L186、N187 到 L217 以及 R221 到 D250 的三个锚蛋白重复序列）和一个 NPR1 样结构域（C262 到 S469）。 NPR1 基因的 DNA 序列为 2,332 或 2,223 bp。两个序列均包含三个内含子和四个外显子。 NPR1转录本主要在根和叶中表达，在茎中检测到的信号较少。水杨酸攻击后 1 小时，NPR1 转录物的量显着增加，最终比对照组高 5.3 倍。 DNA序列和编码序列均从8个花生品种和9个野生品种中获得。对来自不同物种的 25 个序列的 dN/dSratios 最大似然分析表明，不同的选择压力可能作用于不同的分支。
TheNPR1gene was an important regulator for a plant disease resistance. The cDNA ofNPR1gene was cloned from peanut cultivar Ri Hua 1 by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The full length cDNA ofArachis hypogaeaNPR1consisted of 2,078 base pairs with a 1,446 bp open-reading frame encoding 481 amino acids. The predicted NPR1 contained the highly conserved functional domains (BTB/POZ domain from M1 to D116), protein–protein interaction domains (three ankyrin repeats from K158 to L186; N187 to L217 and R221 to D250) and one NPR1-like domain (C262 to S469). The DNA sequence of theNPR1gene was 2,332 or 2,223 bp. Both two sequences contained three introns and four exons. TheNPR1transcripts were expressed mainly in roots and leaves, while fewer signals were detected in the stems. Amount of theNPR1transcript was significantly increased 1 h after salicylic acid challenge and was eventually 5.3 times greater than that in the control group. Both the DNA sequence and the coding sequence were obtained from eight cultivars and nine wild species ofArachis. Maximum likelihood analyses ofdN/dSratios for 25 sequences from different species showed that different selection pressures may have acted on different branches.