Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson's disease and control patients using the micro-XANES technique
Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson's disease and control patients using the micro-XANES technique
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DOI：
10.1007/s00775-006-0179-5
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发表时间：
2007-02-01
影响因子：
3
通讯作者：
Lankosz, Marek
中科院分区：
文献类型：
--
作者：
Chwiej, Joanna;Adamek, Dariusz;Lankosz, Marek
应用 X 射线吸收近边缘结构光谱来研究代表帕金森病 (PD) 的黑质 (SN) 神经细胞与对照病例之间铁化学状态的差异。尸检样本是使用冷冻切片机切割的，未固定且未包埋在石蜡中。 PD 和对照样本的 SN 黑化神经元中测量的铁 K 边缘附近的吸收光谱的比较没有显示出铁氧化态的显着差异。对含有第二和第三氧化态铁的无机标准物质的测量表明，所检查的所有神经细胞体中的大部分铁被氧化并以三价铁（Fe3+）的形式出现。
X-ray absorption near edge structure spectroscopy was applied in order to investigate differences in iron chemical state between the nerve cells of substantia nigra (SN) representing Parkinson's disease (PD) and those of control cases. Autopsy samples were cut using a cryotome, and were not fixed and not embedded in paraffin. The comparison of the absorption spectra near the iron K-edge measured in melanized neurons from SN of PD and control samples did not show significant differences in iron oxidation state. Measurements of inorganic reference materials containing iron in the second and third oxidation states indicate that most of the iron in all the nerve cell bodies examined was oxidized and occurred as trivalent ferric iron (Fe3+).