EXCISION OF THE EN/SPM TRANSPOSABLE ELEMENT OF ZEA-MAYS REQUIRES 2 ELEMENT-ENCODED PROTEINS
EXCISION OF THE EN/SPM TRANSPOSABLE ELEMENT OF ZEA-MAYS REQUIRES 2 ELEMENT-ENCODED PROTEINS
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DOI：
10.1002/j.1460-2075.1990.tb07625.x
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发表时间：
1990-12-01
期刊：
影响因子：
11.4
通讯作者：
GIERL, A
中科院分区：
文献类型：
--
作者：
FREY, M;REINECKE, J;GIERL, A
在转基因烟草中开发了 En/Spm 切除测定系统。切除和整合的特征与玉米的自然系统相似。在该转基因模型系统中，切除需要两个 En/Spm 编码的反式作用功能 TNPA 和 TNPD。提出了转座的生化模型，该模型也可能适用于其他转座元件。
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.