LOCALIZATION OF PEROXIDASE ACTIVITY IN THE DEVELOPING SUBMANDIBULAR GLAND OF NORMAL AND ISOPROTERENOL-TREATED RATS
LOCALIZATION OF PEROXIDASE ACTIVITY IN THE DEVELOPING SUBMANDIBULAR GLAND OF NORMAL AND ISOPROTERENOL-TREATED RATS
复制标题
正常大鼠和异丙肾上腺素治疗大鼠下颌下腺发育中过氧化物酶活性的定位
DOI：
10.1177/20.11.855
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发表时间：
1972
影响因子：
3.2
通讯作者：
T. Barka
中科院分区：
文献类型：
--
作者：
S. Yamashina;T. Barka
使用二氨基联苯胺-H2O2 方法研究了正常大鼠和异丙肾上腺素治疗大鼠发育中的下颌下腺中过氧化物酶活性的定位。在出生后发育过程中，过氧化物酶活性位于腺泡原细胞和腺泡细胞中。原腺细胞的特征是存在多态性分泌颗粒，产生强烈的过氧化物酶反应，特别是在 1 日龄大鼠的腺体中。除了分泌颗粒外，粗面内质网和核膜中也显示出酶活性。终末小管细胞和导管细胞缺乏过氧化物酶活性。完全发育的腺泡细胞中的分泌颗粒显示很少或没有酶活性。异丙肾上腺素刺激腺泡细胞分泌过氧化物酶阳性颗粒。刺激后，过氧化物酶阳性分泌物质重新积累。在此过程中，高尔基复合体中的酶活性得到了证实。异丙肾上腺素对终末小管细胞没有影响。给予毛果芸香碱后，观察到腺泡原细胞分泌颗粒的消耗效果较差。对 5 日龄大鼠长期给予异丙肾上腺素会导致腺泡细胞分化加速和腺体肥大，但过氧化物酶活性的定位没有显着变化。
The localization of peroxidase activity was studied in the developing submandibular gland of normal and isoproterenol-treated rats using a diaminobenzidine-H2O2 method. During postnatal development peroxidase activity was localized in the proacinar and acinar cells. The proacinar cells were characterized by the presence of polymorphic secretory granules that gave a strong peroxidase reaction, particularly in the glands of 1-day-old rats. In addition to the secretory granules, enzyme activity was demonstrated in the rough endoplasmic reticulum and nuclear envelope. The terminal tubule cells and duct cells were devoid of peroxidase activity. The secretory granules in the fully developed acinar cells revealed little or no enzyme activity. Isoproterenol stimulated the secretion of the peroxidase-positive granules from the proacinar cells. The stimulation was followed by a reaccumulation of peroxidase-positive secretory material. During this process enzyme activity was demonstrable in the Golgi complex. Isoproterenol had no effect on the terminal tubule cells. A less effective depletion of the secretory granules from the proacinar cells was seen after pilocarpine administration. Chronic administration of isoproterenol to 5-day-old rats led to an acceleration of the differentiation of acinar cells and to a hypertrophy of the gland, without significant change in the localization of peroxidase activity.