A bZIP transcription factor, LrbZIP1, is involved in Lilium regale Wilson defense responses against Fusarium oxysporum f. sp. lilii
A bZIP transcription factor, LrbZIP1, is involved in Lilium regale Wilson defense responses against Fusarium oxysporum f. sp. lilii
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bZIP 转录因子 LrbZIP1 参与 Lilium regale Wilson 对尖孢镰刀菌 (Fusarium oxysporum f) 的防御反应。
DOI：
10.1007/s13258-014-0214-9
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Chen CY
中科院分区：
文献类型：
--
作者：
Ji B;Han Q;Ge F;Chen CY
碱性亮氨酸拉链（bZIP）蛋白广泛存在于植物中，在植物防御反应中起重要作用。本研究以王百合枯萎病菌的抑制性消减杂交cDNA文库中的一个表达序列标签为基础，对王百合枯萎病菌的致病性进行了研究。在百合黑胫病菌（L. lilii sp.）感染的条件下，从百合黑胫病菌（L. lilii sp.）采用快速扩增cDNA末端的方法，LrbZIP 1蛋白由142个氨基酸残基组成，含有一个碱性结构域和一个亮氨酸拉链基序。定量逆转录PCR（qRT-PCR）分析表明，LrbZIP 1基因在根中的转录水平较高。比在年轻的茎和叶盛宴。LrbZIP 1基因在L. regale和F. oxysporum F. sp. lilii以及用应激相关信号分子处理后。为了验证LrbZIP 1的功能，构建了LrbZIP 1的组成型表达载体，并将其转入烟草（Nicotiana tabacum L.）cv Xanthi）。Southern杂交和qRT-PCR分析结果表明，LrbZIP 1已整合到烟草转化子的基因组中并高效表达。在正常条件下，T1代转基因烟草表现出比野生型更高的抗氧化酶活性和几个抗性相关基因的转录水平。此外，T1代转基因烟草植株对F. oxysporum F.百合疫霉感染。
The basic leucine zipper (bZIP) proteins are ubiquitous in plants and play important roles in plant defense responses. In this study, based on an expressed sequence tag from a suppression subtractive hybridization cDNA library of Lilium regale Wilson during Fusarium oxysporum f. sp. lilii infection, a novel bZIP transcription factor gene LrbZIP1 was isolated from L. regale root using the rapid amplification of cDNA ends method. The predicted protein of LrbZIP1 with 142 amino acid residues contains a basic domain signature and a leucine zipper motif. The quantitative reverse transcription-PCR (qRT-PCR) analysis showed that the transcription level of LrbZIP1 was higher in roots of L. regale than in young stems and leaves. Moreover, the expression of LrbZIP1 was up-regulated in the incompatible interaction between L. regale and F. oxysporum f. sp. lilii as well as after treatments with stress-related signaling molecules. To verify the function of LrbZIP1, a constitutive expression vector of LrbZIP1 was constructed and transferred into tobacco (Nicotiana tabacum L. cv Xanthi). The results of Southern blotting and qRT-PCR analyses demonstrated that the LrbZIP1 was integrated into genome of the tobacco transformants and highly expressed. Under normal conditions, the T1 transgenic tobacco lines showed higher antioxidant enzyme activities and transcription levels of several resistance-related genes than the wild type. Moreover, the T1 transgenic tobacco plants showed strong resistance to F. oxysporum f. sp. lilii infection.
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