Nestin-positive microglia in adult rat cerebral cortex
Nestin-positive microglia in adult rat cerebral cortex
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DOI：
10.1016/j.brainres.2009.03.014
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发表时间：
2009-05
期刊：
影响因子：
2.9
通讯作者：
Y. Takamori;Tetsuji Mori;T. Wakabayashi;Yosuke Nagasaka;T. Matsuzaki;H. Yamada
中科院分区：
文献类型：
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作者：
Y. Takamori;Tetsuji Mori;T. Wakabayashi;Yosuke Nagasaka;T. Matsuzaki;H. Yamada
巢蛋白是一种VI类中间丝蛋白，最早发现于神经系统发育的早期阶段。它被广泛用作干细胞或祖细胞标记物。在成年哺乳动物脑中，巢蛋白不仅在神经源性区域的生发细胞中表达，而且在非生发细胞中也有表达，如反应性星形胶质细胞、内皮细胞和周细胞。在目前的研究中，我们在成年大鼠的大脑皮质中发现了另一种巢蛋白阳性细胞类型。我们用免疫组织化学方法分析了哪些类型的细胞表现出Nestin的免疫反应，并通过与Iba1、CD11b和GLUT5(已知为小胶质细胞特异性)的联合免疫染色，鉴定这些细胞为小胶质细胞。正常情况下，大鼠大脑皮质中约有20%的小胶质细胞呈Nestin免疫反应阳性。巢蛋白信号不广泛分布于小胶质细胞胞浆中，但仅限于核周和部分细胞突起。巢蛋白阳性的小胶质细胞对中间丝蛋白波形蛋白也呈免疫反应。这些观察表明，处于静息状态的小胶质细胞亚群具有含有巢蛋白的中间丝状网络。因此，巢蛋白和波形蛋白可能在维持小胶质细胞的结构完整性方面发挥作用。
Nestin is a class VI intermediate filament protein, which was first identified in the early developmental stages of the nervous system. It is widely used as a stem or progenitor cell marker. In the adult mammalian brain, nestin is expressed not only in germinal cells in the neurogenic regions but also in non-germinal cells, such as reactive astrocytes, endothelial cells and pericytes. In the present study, we found another nestin-positive cell type within the adult rat cerebral cortex. We immunohistochemically analyzed which types of cells exhibit immunoreactivity for nestin, and through the use of co-immunostaining with Iba1, CD11b and GLUT5, which are known to be specific for microglia, identified these cells as microglia. Approximately >20% of the microglia were immunoreactive for nestin in the rat cerebral cortex under normal conditions. Nestin signals were not widely distributed in the microglial cytoplasm, but were restricted to the perikaryon and to parts of the cell processes. Nestin-positive microglia were also immunoreactive for the intermediate filament protein vimentin. These observations demonstrate that a subpopulation of microglia in a resting state has nestin-containing intermediate filament networks. Therefore, nestin in conjunction with vimentin might have roles in maintaining the structural integrity of the microglia.