Identification and expression of a factor of the DM family in the oyster Crassostrea gigas
Identification and expression of a factor of the DM family in the oyster Crassostrea gigas
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DOI：
10.1016/j.cbpa.2008.09.019
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发表时间：
2009-02-01
影响因子：
2.3
通讯作者：
Sourdaine, Pascal
中科院分区：
文献类型：
--
作者：
Naimi, Amine;Martinez, Anne-Sophie;Sourdaine, Pascal
太平洋牡蛎Crassostrea gias是一种连续的、不系统的原性两性体。在寻找与DMRT1同源的保守性决定论因子DMRT1的基础上，我们首次在细毛虫中发现了一个DM因子的完整cDNA，我们称之为CG-DML(Crassostrea Gigas DMRT-like)。它长359aa，具有所有家族因子共有的DM结构域，以及一个特定于Dmrt4和Dmrt5等成员的DMA结构域。它的基因存在一个598bp的内含子。实时荧光定量聚合酶链式反应和原位杂交结果表明，CG-DML在两性中均有表达，在成体配子发生周期结束时，雄性性腺的表达显著高于雌性性腺，在1~2个月龄的SpA中表达达到显著高峰。这些结果提示CG-DML可能参与了性腺的发育，并可能为今后的工作提供初步线索，以便更好地了解DM蛋白的进化。(C)2008 Elsevier Inc.保留所有权利。
The Pacific oyster Crassostrea gigas is a successive not systematic protandric hermaphrodite. Searching for an ortholog to Dmrt1, a conserved sex determinism factor, we have identified the first complete cDNA of a DM factor in Lophotrochozoa which we have called Cg-DMl (Crassostrea gigas DMRT-like). It is 359aa long, with the DM domain common to all the family factors, and one DMA domain specific to members such as Dmrt4 and Dmrt5. Its gene presents one intron of 598 bp. Real time PCR and in situ hybridization have shown that Cg-DMl was expressed in both sexes, with a significantly higher expression in male than in female gonads at the end of the adult gametogenetic cycle and that a significant peak of expression was observed in spat between 1 and 2 months of age. These results suggest that Cg-DMl may be involved in the development of the gonad and may constitute preliminary clues for future work in order to better understand DM protein evolution. (C) 2008 Elsevier Inc. All rights reserved.