Protein:protein interactions and the pairing of boundary elements in vivo
Protein:protein interactions and the pairing of boundary elements in vivo
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DOI：
10.1101/gad.1052003
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发表时间：
2003-03-01
影响因子：
10.5
通讯作者：
Schedl, P
中科院分区：
文献类型：
--
作者：
Blanton, J;Gaszner, M;Schedl, P
虽然它是现在公认的边界元件/绝缘子的功能，真核细胞染色体细分为自主调控域，潜在的机制仍然难以捉摸。一种观点认为，边界起着屏障的作用，阻止了“活跃”或“沉默”的染色质在结构域之间的进行性扩散。另一种观点认为，划分为自主功能单位是染色体潜在结构细分为高阶“环状”结构域的结果。在这种观点中，边界被认为是通过相互作用或与其他核结构相互作用来界定结构域。这里报道的研究提供了支持的环状结构域模型。我们表明，果蝇scs和scs的边界蛋白，Zw 5和BEAF，分别在体外和体内相互作用。此外，与这种蛋白质：蛋白质相互作用可能促进边界元件配对的想法一致，我们发现，在果蝇细胞核中，scs和scs'彼此非常接近。
Although it is now well-established that boundary elements/insulators function to subdivide eukaryotic chromosomes into autonomous regulatory domains, the underlying mechanisms remain elusive. One idea is that boundaries act as barriers, preventing the processive spreading of "active" or "silenced" chromatin between domains. Another is that the partitioning into autonomous functional units is a consequence of an underlying structural subdivision of the chromosome into higher order "looped" domains. In this view, boundaries are thought to delimit structural domains by interacting with each other or with some other nuclear structure. The studies reported here provide support for the looped domain model. We show that the Drosophila scs and scs' boundary proteins, Zw5 and BEAF, respectively, interact with each other in vitro and in vivo. Moreover, consistent with idea that this protein:protein interaction might facilitate pairing of boundary elements, we find that that scs and scs' are in close proximity to each other in Drosophila nuclei.