Particle display: a quantitative screening method for generating high-affinity aptamers.
Particle display: a quantitative screening method for generating high-affinity aptamers.
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DOI：
10.1002/anie.201309334
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发表时间：
2014-05-05
影响因子：
16.6
通讯作者：
Soh, H. Tom
中科院分区：
文献类型：
--
作者：
Wang, Jinpeng;Gong, Qiang;Maheshwari, Nupur;Eisenstein, Michael;Arcila, Mary Luz;Kosik, Kenneth S.;Soh, H. Tom
我们报告了一种适体发现技术，与传统选择相比，该技术可以在更少的轮次中重复产生更高亲和力的适体。我们的方法（称为颗粒显示）将溶液相适体库转化为“适体颗粒”，每个颗粒在其表面上显示单个序列的许多副本。然后，我们使用荧光激活细胞分选 (FACS) 单独测量 > 108 个适体颗粒的相对亲和力，并以高通量方式对它们进行分选。通过数学分析，我们确定了能够实现最佳筛选的实验参数，并证明了富集性能超出了传统选择可实现的理论最大值多个数量级。我们利用粒子展示技术，在三轮中获得了针对四种不同蛋白质靶标的高亲和力DNA适体，其中包括之前DNA适体选择工作未成功的蛋白质。我们相信，粒子展示提供了一种非常有效的机制，可以快速、经济地生成高质量的适体，从而加速人类蛋白质组的探索。
We report an aptamer discovery technology that reproducibly yields higher affinity aptamers in fewer rounds compared to conventional selection. Our method (termed particle display) transforms libraries of solution-phase aptamers into “aptamer particles”, each displaying many copies of a single sequence on its surface. We then use fluorescence-activated cell sorting (FACS) to individually measure the relative affinities of >108 aptamer particles and sort them in a high-throughput manner. Through mathematical analysis, we identified experimental parameters that enable optimal screening, and demonstrate enrichment performance that exceeds the theoretical maximum achievable with conventional selection by many orders of magnitude. We used particle display to obtain high-affinity DNA aptamers for four different protein targets in three rounds, including proteins for which previous DNA aptamer selection efforts have been unsuccessful. We believe particle display offers an extraordinarily efficient mechanism for generating high quality aptamers in a rapid and economic manner, towards accelerated exploration of the human proteome.
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影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
影响因子：
3.7
作者：
Ahmad KM;Oh SS;Kim S;McClellen FM;Xiao Y;Soh HT
通讯作者：
Soh HT
影响因子：
5.6
作者：
Tasset, DM;Kubik, MF;Steiner, W
通讯作者：
Steiner, W
影响因子：
5.6
作者：
IRVINE, D;TUERK, C;GOLD, L
通讯作者：
GOLD, L
影响因子：
15.9
作者：
McNamara, James O., II;Kolonias, Despina;Gilboa, Eli
通讯作者：
Gilboa, Eli