Oct4 Expression in Immature Teratoma of the Ovary Relevance to Histologic Grade and Degree of Differentiation
Oct4 Expression in Immature Teratoma of the Ovary Relevance to Histologic Grade and Degree of Differentiation
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DOI：
10.1097/pas.0b013e3181fcd707
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发表时间：
2010-12-01
影响因子：
5.6
通讯作者：
Konishi, Ikuo
中科院分区：
文献类型：
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作者：
Abiko, Kaoru;Mandai, Masaki;Konishi, Ikuo
卵巢未成熟畸胎瘤是一种罕见的肿瘤，占卵巢恶性肿瘤的1%。畸胎瘤中未成熟神经上皮细胞的数量是一个重要的预后因素。尽管目前基于这一标准的分级系统已被广泛接受，但未成熟神经上皮的生物学意义却鲜为人知。在本研究中，我们用免疫组织化学方法检测了18例卵巢未成熟畸胎瘤中Oct4(也称为Oct3或Pou5f1)、PAX6和CD56的表达。Oct4是一种在原始生殖细胞和胚胎干细胞中表达的转录因子，PAX6是一种促进神经发生的转录因子，CD56是神经源性肿瘤的已知标志物。Oct4在7例3级和2例2级未成熟神经上皮中均有表达。4例2级和5例1级均不表达。这些肿瘤细胞缺乏CD30或甲胎蛋白表达，这支持纯未成熟畸胎瘤的诊断。Pax6在所有未成熟畸胎瘤的未成熟神经上皮细胞中均有表达，但在Oct4阳性细胞中不表达。CD56在不同成熟度的神经成分中表达，包括PAX6阳性的未成熟神经上皮，而在Oct4阳性的细胞中不表达。提示这些标记物的表达可能反映了未成熟畸胎瘤神经组织的分化状态。Oct4仅在高度恶性未成熟畸胎瘤的未成熟神经上皮细胞中表达，提示Oct4可能成为诊断高度恶性的未成熟畸胎瘤的一种有前途的生物标志物。
Immature teratoma of the ovary is an uncommon tumor comprising 1% of ovarian malignancies. The amount of immature neuroepithelium in the teratoma is an important prognostic factor. Although the current grading system based on this criterion is widely accepted, the biological significance of immature neuroepithelium is poorly understood. In this study, we used immunohistochemistry to evaluate the expression of Oct4 (also known as Oct3 or POU5F1), a transcription factor expressed in primordial germ cells and embryonic stem cells, along with that of PAX6, a transcription factor contributing to neurogenesis, and CD56, a known marker for tumors of neural origin, in 18 cases of pure immature teratoma of the ovary. Oct4 was expressed in the immature neuroepithelium of all 7 grade-3 cases and 2 grade-2 cases. It was not expressed in 4 grade-2 cases and all 5 grade-1 cases. These tumor cells lacked CD30 or alpha-fetoprotein expression, which supported the diagnosis of pure immature teratoma. PAX6 was expressed in the immature neuroepithelium of all immature teratomas, but not in Oct4-positive cells. CD56 was expressed in neural components of various maturities including PAX6-positive immature neuroepithelium, but not in Oct4-positive cells. These data suggest that the expression of these markers probably reflects the differentiation status of neural tissue in immature teratomas. The finding that Oct4 expression was exclusively detected in immature neuroepithelium of high-grade immature teratomas indicates that Oct4 might serve as a promising biomarker for the diagnosis of highly malignant cases of immature teratoma.