Borrelia burgdorferi changes its surface antigenic expression in response to host immune responses
Borrelia burgdorferi changes its surface antigenic expression in response to host immune responses
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DOI：
10.1128/iai.72.10.5759-5767.2004
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
Liang, FT;Yan, J;Fikrig, E
莱姆病螺旋体，伯氏疏螺旋体，导致持续的哺乳动物感染，尽管对病原体的强烈免疫反应的发展。为了研究在不利的免疫环境中占主导地位的螺旋体表型，在各种免疫条件下通过定量逆转录-PCR分析了四种原型表面脂蛋白、核心蛋白聚糖结合蛋白A（DbpA）、外表面蛋白C（OspC）、BBF 01和VlsE的mRNA转录。我们证明了B. burgdorferi改变其表面抗原表达以响应免疫攻击。dbpA表达不变，而螺旋体使ospC表达降低了446倍，并使BBF 01和vlsE表达分别增加了20和32倍，这是在感染期间免疫感受态小鼠中产生的免疫压力的影响下。抗原表达的这种变化可以通过用特异性疏螺旋体抗血清或OspC抗体被动免疫感染的严重联合免疫缺陷小鼠来诱导，并且似乎允许B。以抵抗免疫攻击。
The Lyme disease spirochete, Borrelia burgdorferi, causes persistent mammalian infection despite the development of vigorous immune responses against the pathogen. To examine spirochetal phenotypes that dominate in the hostile immune environment, the mRNA transcripts of four prototypic surface lipoproteins, decorin-binding protein A (DbpA), outer surface protein C (OspC), BBF01, and VlsE, were analyzed by quantitative reverse transcription-PCR under various immune conditions. We demonstrate that B. burgdorferi changes its surface antigenic expression in response to immune attack. dbpA expression was unchanged while the spirochetes decreased ospC expression by 446 times and increased BBF01 and vlsE expression up to 20 and 32 times, respectively, under the influence of immune pressure generated in inummocompetent mice during infection. This change in antigenic expression could be induced by passively immunizing infected severe combined immunodeficiency mice with specific Borrelia antisera or OspC antibody and appears to allow B. burgdorferi to resist immune attack.