Close Association of CD8+/CD38bright with HIV-1 Replication and Complex Relationship with CD4+ T-Cell Count
Close Association of CD8+/CD38bright with HIV-1 Replication and Complex Relationship with CD4+ T-Cell Count
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DOI：
10.1002/cyto.b.20467
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发表时间：
2009-07-01
影响因子：
3.4
通讯作者：
Vendrell, Jean-Pierre
中科院分区：
文献类型：
--
作者：
Tuaillon, Edouard;Al Tabaa, Yassine;Vendrell, Jean-Pierre
背景：除了 CD4(+) T 细胞计数之外，测量淋巴细胞活化还可以为 HIV-1 感染患者的免疫监测提供信息。 CD38 是一种成熟的激活标记物，通常在整个 CD8(+) T 细胞群上进行分析。特别关注 CD38 高表达 (CD8(+)/CD38(bright)) 可能是一种有趣的替代门控策略，因为 CD38(bright) 主要表征激活的记忆细胞。方法：在一年的时间内对 1,353 名 HIV-1 感染患者进行了 CD8(+)/CD38(bright) 的研究，以建立相关的截止值并阐明该标记物与 HIV-1 RNA 病毒载量 (VL) 和 CD4(+) 的关系T 细胞计数。结果：在对开始抗病毒治疗 (ART) 的非免疫抑制患者进行的纵向随访中，CD8(+)/CD38（亮）（每个细胞 >8,500 个 CD38 结合位点）与 HIV-1 VL 密切相关（r = 0.87，P < 0.001）。在接受 ART 的无病毒血症患者中，该标志物高度预测 VL 反弹（对于 VL 检测水平 >200 拷贝/ml，敏感性为 93%，特异性为 64%）。虽然 CD8(+)/CD38(bright) 与 CD4(+) T 细胞计数呈中等相关，与 VL 无关（r = -0.37，P < 0.001），但在表现出深度 CD4(+) T 细胞耗竭的无病毒血症患者组中，CD8(+)/CD38(bright) 显着增加（CD4(+) T 细胞计数中位数为 39%
Background: Measuring lymphocyte activation provides information in addition to CD4(+) T-cell count for immune monitoring of HIV-1 infected patients. CD38 is a well-established activation marker that is generally analyzed on the whole population of CD8(+) T-cells. Focusing specifically on CD38 high expression (CD8(+)/CD38(bright)) may be an interesting surrogate gating strategy because CD38(bright) characterizes principally activated memory cells.Methods: CD8(+)/CD38(bright) was investigated in 1,353 HIV-1 infected patients over a one-year period to establish relevant cutoff values and clarify the relationships of this marker with HIV-1 RNA viral load (VL) and CD4(+) T-cell count.Results: The CD8(+)/CD38(bright) (>8,500 CD38 binding site per cells) is well correlated with HIV-1 VL (r = 0.87, P < 0.001) in this longitudinal follow-up of nonimmunodepressed patients that initiated antiviral therapy (ART). In aviremic patients on ART, the marker was highly predictive of VL rebound (sensitivity 93%, specificity 64% for a VL level of detection >200 copies/ml). While the CD8(+)/CD38(bright) moderately correlated with CD4(+) T-cell count independently of the VL (r = -0.37, P < 0.001), it increased dramatically in aviremic patient groups that exhibited profound CD4(+) T-cell depletion (median 39% for CD4(+) T-cell counts