Modulation of cytokines, resistin, and distribution of adipose tissue in C57BL/6 mice by different high-fat diets
Modulation of cytokines, resistin, and distribution of adipose tissue in C57BL/6 mice by different high-fat diets
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DOI：
10.1016/j.nut.2011.05.011
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发表时间：
2012-02-01
期刊：
影响因子：
4.4
通讯作者：
Aguila, Marcia Barbosa
中科院分区：
文献类型：
--
作者：
Catta-Preta, Mariana;Martins, Marcela Anjos;Aguila, Marcia Barbosa
目的：探讨改变脂质来源是否会引起高脂饮食小鼠代谢变化和/或调节脂肪组织分布。方法：C57BL/6小鼠分别饲喂10周的对照饲粮（10%脂肪）或含猪油（HF- l）、橄榄油（HF- o）、葵花籽油或菜籽油的HF饲粮（60%脂肪）。测量食物摄入量和体重。在安乐死时，采集血液并解剖脂肪组织。测定血清激素和细胞因子。结果：HF-L组和HF-O组血浆胰岛素水平高于其他3组（P < 0.0001）。抵抗素水平以HF-L组和HF-O组最高（P < 0.0001）。两组瘦素表达量最高（P < 0.0001）。四组中，HF-L组白细胞介素-6表达水平最高（P < 0.0005），脂联素表达水平最低（P < 0.0001）。与其他组相比，HF-L组皮下和内脏脂肪组织的积累更高。由于脂肪细胞中皮下脂肪和附睾脂肪过多，本组呈肥厚。然而，与其他组相比，HF-L和HF-O组皮下脂肪与内脏脂肪的比例显著降低。结论：高脂饮食对小鼠脂肪因子水平、脂肪组织分布及碳水化合物代谢的影响更明显地受脂肪含量的影响，而非脂肪的绝对含量。(C) 2012爱思唯尔公司版权所有。
Objective: To investigate whether changing the lipid source induces metabolic changes and/or modulates the adipose tissue distribution in mice fed with a high-fat (HF) diet.Methods: C57BL/6 mice were subjected to a 10-wk control diet (10% fat) or an HF diet (60% fat) containing lard (HF-L), olive oil (HF-O), sunflower oil, or canola oil. Food intake and body weight were measured. At euthanasia, blood was collected and adipose tissue was dissected. Serum hormones and cytokines were determined.Results: The plasma insulin levels were higher in the HF-L and HF-O groups than in the other three groups (P < 0.0001). The levels of resistin were highest in the HF-L and HF-O groups (P < 0.0001). Leptin expression was also highest in these two groups (P < 0.0001). Of the four groups, interleukin-6 was expressed at the highest level in the HF-L group (P < 0.0005), whereas adiponectin was expressed at the lowest level (P < 0.0001). The accumulation of subcutaneous and visceral adipose tissues was higher in the HF-L group compared with the other groups. This group was hypertrophic because of excess subcutaneous fat and epididymal fat in the adipocytes. However, the ratio of subcutaneous to visceral fat was significantly lower in the HF-L and HF-O groups compared with the other groups.Conclusion: In mice fed fat-rich diets, the level of adipokines, the distribution of adipose tissue, and the metabolism of carbohydrates are more significantly influenced by the lipid content rather than the absolute amount of lipid. (C) 2012 Elsevier Inc. All rights reserved.