Heixuedian (heix), a potential melanotic tumor suppressor gene, exhibits specific spatial and temporal expression pattern during Drosophila hematopoiesis.
Heixuedian (heix), a potential melanotic tumor suppressor gene, exhibits specific spatial and temporal expression pattern during Drosophila hematopoiesis.
复制标题
DOI：
10.1016/j.ydbio.2014.12.001
复制
发表时间：
2015-02
影响因子：
2.7
通讯作者：
Yan Xia;S. Z. Midoun;Zhiliang Xu;Ling Hong
中科院分区：
文献类型：
--
作者：
Yan Xia;S. Z. Midoun;Zhiliang Xu;Ling Hong
TheDrosophila heixuedian(heix) is the ortholog of humanUBIAD1gene (a.k.aTERE1). The protein product ofUBIAD1/heixhas multiple enzymatic activities, including the vitamin K2 and the non-mitochondrial CoQ10 biosynthesis. However, the expression pattern of UBIAD1/Heix during metazoan development has not been systematically studied. In this paper, we found that loss of function ofheixresulted in pathological changes of larval hematopoietic system, including lymph gland hypertrophy, hemocyte overproliferation and aberrant differentiation, and melanin mass formation. Overexpression ofheixcDNA under the tubulin Gal4 driver rescued the above hematopoietic defects. Interestingly, Heix was specifically expressed in plasmatocyte/macrophage lineage in srp driven EGFP positive cells on the head mesoderm during embryogenesis, while it was highly expressed in crystal cells in the primary lobes of the third instar larval lymph gland. Using qRT-PCR analysis, loss of function ofheixcaused aberrant activation of multiple hemocyte proliferation-related as well as immune-related pathways, including JAK/STAT pathway, Ras/MAPK pathway, IMD pathway and Toll pathway. These data suggested thatheixis a potential melanotic tumor suppressor gene and plays a pivotal role in both hemocytes proliferation and differentiation inDrosophila.
