Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)
Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros)
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DOI：
10.1016/j.ibmb.2016.02.004
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发表时间：
2016-04-01
影响因子：
3.8
通讯作者：
Siegfried, Blair D.
中科院分区：
文献类型：
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作者：
Fishilevich, Elane;Velez, Ana M.;Siegfried, Blair D.
RNA干扰（RNAi）是一种存在于动物和植物中的基因沉默机制，由双链RNA（dsRNA）或小干扰RNA（siRNA）触发，具体取决于生物体。在西方玉米根虫（WCR），玉米根萤叶甲（Diabrotica virgifera virgifera LeConte）（鞘翅目：玉米根萤叶甲科）中，RNAi可以通过向根虫饲喂添加到人工饲料或转化以表达dsRNA的植物组织中的dsRNA来实现。RNAi的效果取决于靶基因的功能，并且可以从表型反应的缺乏到明显的反应，包括致死性。此外，RNAi可以直接影响消耗dsRNA的个体，或者这种影响可以转移到下一代。我们先前的工作描述了参与胚胎发育的基因作为控制WCR的亲本RNAi技术的潜在用途。在这项研究中，我们描述了使用染色质重塑ATP酶作为靶基因，以实现亲本基因沉默的两种害虫，鞘翅目，WCR，和半翅目，新热带区的棕色臭虫，英雄美洲蝽（半翅目：蝽科）。我们的研究结果表明，双链RNA靶向染色质重塑ATP酶的成绩单，brahma，mi-2，和iswi强烈降低了这两种昆虫物种中暴露的女性的生殖力。此外，敲低chd 1基因降低了E.英雄(C)2016爱思唯尔有限公司版权所有
RNA interference (RNAi) is a gene silencing mechanism that is present in animals and plants and is triggered by double stranded RNA (dsRNA) or small interfering RNA (siRNA), depending on the organism. In the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), RNAi can be achieved by feeding rootworms dsRNA added to artificial diet or plant tissues transformed to express dsRNA. The effect of RNAi depends on the targeted gene function and can range from an absence of phenotypic response to readily apparent responses, including lethality. Furthermore, RNAi can directly affect individuals that consume dsRNA or the effect may be transferred to the next generation. Our previous work described the potential use of genes involved in embryonic development as a parental RNAi technology for the control of WCR. In this study, we describe the use of chromatin-remodeling ATPases as target genes to achieve parental gene silencing in two insect pests, a coleopteran, WCR, and a hemipteran, the Neotropical brown stink bug, Euschistus heros Fabricius (Hemiptera: Pentatomidae). Our results show that dsRNA targeting chromatin-remodeling ATPase transcripts, brahma, mi-2, and iswi strongly reduced the fecundity of the exposed females in both insect species. Additionally, knockdown of chd1 reduced the fecundity of E. heros. (C) 2016 Elsevier Ltd. All rights reserved.