Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41.
Yeast Mitochondrial Transcription Factor Mtf1 Determines the Precision of Promoter-Directed Initiation of RNA Polymerase Rpo41.
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DOI：
10.1371/journal.pone.0136879
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yin YW
中科院分区：
文献类型：
--
作者：
Yang X;Chang HR;Yin YW
尽管它们明确的T7噬菌体起源，线粒体RNA聚合酶已经进化到需要转录因子。所有线粒体聚合酶都含有一个额外的N末端结构域，该结构域在自精通噬菌体酶中没有对应物，因此假设该结构域与转录因子相互作用。我们研究了一系列酵母线粒体RNA聚合酶Rpo 41的N-末端缺失突变体，发现N-末端区域并不消除Mtf 1的作用;相反，它直接有助于酶催化。Mtf 1可以拯救由N端结构域缺失导致的缺陷Rpo 41酶。虽然Rpo 41似乎保留了T7 RNAP中发现的所有启动子识别元件，但这些元件不是独立功能的，Mtf 1是全酶启动子指导的转录活性所必需和足够的。
Despite their clear T7-bacteriophage origin, mitochondrial RNA polymerases have evolved to require transcription factors. All mitochondrial polymerases contain an extra N-terminal domain that has no counterpart in the self-proficient phage enzyme, which is therefore hypothesized to interact with transcription factors. We studied a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and have found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis. Mtf1 can rescue the defective Rpo41 enzymes resulted from N-terminal domain deletions. Although Rpo41 appears to have retained all promoter recognition elements found in T7 RNAP, the elements are not independently functional, and Mtf1 is necessary and sufficient for holoenzyme promoter-directed transcription activity.