Collagen-GAG substrate enhances the quality of nerve regeneration through collagen tubes up to level of autograft
Collagen-GAG substrate enhances the quality of nerve regeneration through collagen tubes up to level of autograft
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DOI：
10.1006/exnr.1998.6955
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发表时间：
1998-12-01
影响因子：
5.3
通讯作者：
Spector, M
中科院分区：
文献类型：
--
作者：
Chamberlain, LJ;Yannas, IV;Spector, M
使用组织形态计量学和诱发动作电位的 A 纤维、B 纤维和 C 纤维峰值的电生理学测量，研究大鼠坐骨神经中 10 毫米管状间隙的周围神经再生。由大孔胶原蛋白（最大孔径，22 nm）、小孔胶原蛋白（最大孔径，4 nm）和硅胶制成的管被植入盐水填充或填充高孔隙度胶原-糖胺聚糖（CG）基质。基于之前的优化研究，特意合成了 CG 基质，其降解半衰期约为 6 周，并通过高孔体积分数 (0.95) 和相对较小的平均孔径 (35 μ m) 的组合而具有非常高的比表面。通过由大孔胶原蛋白制成并填充 CG 基质的管再生的神经比任何其他管状组具有明显更多的大直径轴突、更多的总轴突和明显更高的 A 纤维传导速度；而且，尽管低于正常值，但它们的组织形态学和电生理学特性在统计学上与自体移植物对照没有区别。尽管通过管状再生的神经中有髓鞘轴突的总数在 30 周时已达到稳定水平，但直径大于 6 μm 的轴突数量（与动作电位的 A 纤维峰值独特相关）在研究完成时（60 周）继续大幅增加。动力学数据强烈表明，神经干成熟过程在损伤后持续超过 60 周，导致神经干越来越接近正常对照的结构，这一过程在大鼠坐骨神经管状 10 毫米间隙的研究中没有报道过，并且包括轴突组织面积的增加和总组织面积的减少。 (C) 1998 年学术出版社。
Peripheral nerve regeneration was studied across a tubulated 10-mm gap in the rat sciatic nerve using histomorphometry and electrophysiological measurements of A-fiber, B-fiber, and C-fiber peaks of the evoked action potentials. Tubes fabricated from large-pore collagen (max. pore diameter, 22 nm), small-pore collagen (max. pore diameter, 4 nm), and silicone were implanted either saline-filled or filled with a highly porous, collagen-glycosaminoglycan (CG) matrix. The CG matrix was deliberately synthesized, based on a previous optimization study, to degrade with a half-life of about 6 weeks and to have a very high specific surface through a combination of high pore volume fraction (0.95) and relatively small average pore diameter (35 mu m). Nerves regenerated through tubes fabricated from large-pore collagen and filled with the CG matrix had significantly more large-diameter axons, more total axons, and significantly higher A-fiber conduction velocities than any other tubulated group; and, although lower than normal, their histomorphometric and electrophysiological properties were statistically indistinguishable from those of the autograft control. Although the total number of myelinated axons in nerves regenerated by tubulation had reached a plateau by 30 weeks, the number of axons with diameter larger than 6 mu m, which have been uniquely associated with the A-fiber peak of the action potential, continued to increase at substantial rates through the completion of the study (60 weeks). The kinetic data strongly suggest that a nerve trunk maturation process, not previously reported in studies of the tubulated 10-mm gap in the rat sciatic nerve, and consisting in increase of axonal tissue area with decrease in total tissue area, continues beyond 60 weeks after injury, resulting in a nerve trunk which increasingly approaches the structure of the normal control. (C) 1998 Academic Press.