Dibenz [a,j]acridine: distributions of metabolites formed by liver and lung microsomes from control and pretreated rats.
Dibenz [a,j]acridine: distributions of metabolites formed by liver and lung microsomes from control and pretreated rats.
复制标题
二苯并[a,j]吖啶：对照和预处理大鼠的肝和肺微粒体形成的代谢物的分布。
DOI：
10.1093/carcin/8.3.425
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发表时间：
1987
期刊：
影响因子：
4.7
通讯作者：
G. Holder
中科院分区：
文献类型：
--
作者：
J. Gill;C. Duke;A. J. Ryan;G. Holder
The structures of many dibenz[a,j]acridine (DBAJAC) metabolites formed in vitro in incubations with liver microsomes prepared from 3-methylcholanthrene-pretreated male Wistar rats have previously been determined; they were trans-DBAJAC-3,4-dihydrodiol, trans-DBAJAC-5,6-dihydrodiol, DBAJAC-5,6-oxide, 3-hydroxy-DBAJAC, 4-hydroxy-DBAJAC and several multiply oxidized secondary metabolites. Herein are reported [14-3H]DBAJAC metabolite distributions obtained by h.p.l.c. separation of products produced in incubations with liver and lung microsomes prepared from untreated, phenobarbital-pretreated and 3-methylcholanthrene-pretreated male Wistar rats. Liver microsomal metabolites were also quantitated in preparations from trans-stilbene oxide-pretreated rats. For all preparations trans-DBAJAC-3,4-dihydrodiol, the candidate proximate carcinogen according to the bay-region theory of carcinogenesis, was the major metabolite (30-40%) while DBAJAC-5,6-oxide and phenols were also quantitatively important. In incubations conducted in the presence of 3,3,3-trichloropropene-1,2-oxide (1.5 mM) formation of dihydrodiol was inhibited by about 85%. DBAJAC-N-oxide was also identified as a minor metabolite (approximately 1%) formed in incubations with phenobarbital-induced and control liver microsomes.
