Follistatin rescues the inhibitory effect of activin A on the differentiation of bovine preadipocyte
Follistatin rescues the inhibitory effect of activin A on the differentiation of bovine preadipocyte
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DOI：
10.1016/j.domaniend.2006.06.001
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发表时间：
2007-10-01
影响因子：
2.1
通讯作者：
Yano, Hideo
中科院分区：
文献类型：
--
作者：
Hirai, Shizuka;Matsumoto, Haruka;Yano, Hideo
我们研究了激活素A和卵泡抑素对牛前脂肪细胞分化的影响。从约30月龄的日本黑阉牛肾周脂肪组织中制备含有前脂肪细胞的基质血管（SV）细胞。融合后，用1-甲基-3-异丁基-黄嘌呤、地塞米松、胰岛素和曲格列酮诱导分化2天，然后随后培养6天。在分化诱导期间（分化的早期阶段）或在整个分化期用激活素A处理细胞。我们测量了终末分化标志物，如甘油-3-磷酸脱氢酶（GPDH）活性，脂质积累和脂肪细胞脂肪酸结合蛋白mRNA的表达在培养结束时。激活素A抑制了所有分化标志物的诱导，无论治疗持续时间如何。用激活素A处理还降低了过氧化物酶体增殖物激活受体（PPAR）γ和CCAAT/增强子结合蛋白（C/EBP）α mRNA的表达，而不影响C/EBP β mRNA的表达。我们还观察到，卵泡抑素完全拯救激活素A对牛前脂肪细胞分化的抑制作用。此外，与既不用激活素A也不用卵泡抑素处理的细胞相比，即使在激活素A存在下，更高剂量的卵泡抑素也增加了GPDH活性。此外，SV细胞表达激活素A和肌生长抑制素mRNA。这些结果表明，激活素A通过影响转录级联上游的PPAR γ和C/EBP α表达抑制牛前脂肪细胞分化，卵泡抑素抑制激活素A对牛前脂肪细胞分化的抑制作用。内源性激活素A和/或肌肉生长抑制素可能抑制牛前脂肪细胞的分化。(c)2006年爱思唯尔公司All rights reserved.
We investigated the effect of activin A and follistatin on the differentiation of bovine preadipocytes. Stromal-vascular (SV) cells containing preadipocytes were prepared from perirenal adipose tissue of approximately 30-month-old Japanese Black steers. After confluence, differentiation was induced by 1-methyl-3-isobutyl-xanthine, dexamethasone, insulin and troglitasone for 2 days, and then subsequently cultured for 6 days. The cells were treated with activin A during the induction of differentiation (the early phase of differentiation) or throughout the differentiation period. We measured the terminal differentiation markers such as glycerol-3-phosphate dehydrogenase (GPDH) activity, lipid accumulation, and the expression of adipocyte fatty acid-binding protein mRNA at the end of cultures. Activin A suppressed the induction of all differentiation markers regardless of the duration of treatment. The treatment with activin A also reduced the expression of peroxisome proliferator-activated receptor (PPAR) gamma and CCAAT/enhancer binding protein (C/EBP) alpha mRNAs without affecting the expression of C/EBP beta mRNA. We also observed that follistatin completely rescued the inhibitory effect of activin A on bovine preadipocyte differentiation. Furthermore, the higher doses of follistatin increased GPDH activity even in the presence of activin A compared with the cells treated with neither activin A nor follistatin. Additionally, the SV cells expressed activin A and myostatin mRNAs. These results suggest that activin A inhibits bovine preadiopocyte differentiation via affecting transcriptional cascade upstream of PPAR gamma and C/EBP alpha expressions, and that follistatin suppresses the inhibitory effect of activin A on bovine preadipocyte differentiation. Endogenous activin A and/or myostatin possibly inhibit the differentiation of bovine preadipocytes. (c) 2006 Elsevier Inc. All rights reserved.