Mutant identification and characterization of the laccase gene family in Arabidopsis
Mutant identification and characterization of the laccase gene family in Arabidopsis
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DOI：
10.1093/jxb/erl022
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发表时间：
2006-08-01
影响因子：
6.9
通讯作者：
Wu, Yajun
中科院分区：
文献类型：
--
作者：
Cai, Xiaoning;Davis, Elizabeth J.;Wu, Yajun
漆酶，EC 1.10.3.2或对-联苯酚：二氧氧化还原酶，是含多个铜的糖蛋白。尽管多年的研究，漆酶在植物中的作用的遗传证据大多缺乏。在这项研究中，反向遗传学的方法，以确定T-DNA插入突变体（SALK收集）的基因在拟南芥漆酶家族。在该家族的17个漆酶基因（AtLAC 1 ~ AtLAC 17）中，有12个漆酶基因被确认为20个真无效突变体。通过检测鉴定的突变体，发现代表三个漆酶基因突变的四个突变体显示改变的表型。突变体AtLAC 2，lac 2，表现出受损的根伸长PEG诱导的脱水条件下，lac 8开花早于野生型植物，和lac 15表现出改变种子颜色。不同的表型表明，漆酶在植物中执行不同的功能，并不像以前认为的那样是遗传冗余的。这些突变体将被证明是了解体内漆酶功能的宝贵资源。
Laccases, EC 1.10.3.2 or p-diphenol:dioxygen oxidoreductases, are multi-copper containing glycoproteins. Despite many years of research, genetic evidence for the roles of laccases in plants is mostly lacking. In this study, a reverse genetics approach was taken to identify T-DNA insertional mutants (the SALK collection) available for genes in the Arabidopsis laccase family. Twenty true null mutants were confirmed for 12 laccase genes of the 17 total laccase genes (AtLAC1 to AtLAC17) in the family. By examining the mutants identified, it was found that four mutants, representing mutations in three laccase genes, showed altered phenotypes. Mutants for AtLAC2, lac2, showed compromised root elongation under PEG-induced dehydration conditions; lac8 flowered earlier than wild-type plants, and lac15 showed an altered seed colour. The diverse phenotypes suggest that laccases perform different functions in plants and are not as genetically redundant as previously thought. These mutants will prove to be valuable resources for understanding laccase functions in vivo.