Single-shot two-echo technique for simultaneous measurement of GABA and creatine in the human brain in vivo
Single-shot two-echo technique for simultaneous measurement of GABA and creatine in the human brain in vivo
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DOI：
10.1002/mrm.20082
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发表时间：
2004-06-01
影响因子：
3.3
通讯作者：
Shen, J
中科院分区：
文献类型：
--
作者：
Choi, IY;Lee, SP;Shen, J
开发了一种用于同时检测多量子（MQ）过滤的γ-氨基丁酸（GABA）和肌酸（Cr）的单次激发，两次回波方法，并在3特斯拉的人体大脑中进行了演示。同时测量的Cr单线态用作GABA光谱相位的导航器和测量期间由于静磁场（B-0）漂移或受试者移动引起的任何频移，以及内部浓度参考。此外，对GABA的C-3和C-4亚甲基质子使用双波段频率选择性MO滤波器提供了非常稳健的GABA测量，在每次扫描中对重叠代谢物如Cr和谷胱甘肽（GSH）具有极好的抑制作用。重叠大分子的污染也被证明是可以忽略不计的。GABA与Cr的比值为0.09 +/- 0.03（平均值+/- SD，N = 17），体内人脑额顶叶区GABA的估计浓度为0.66 +/- 0.19 μ mol/g（平均值+/- SD，N = 17），外标法为0.69 +/- 0.18 mumol/g（平均值+/- SD，N = 17）。所有受试者中观察到的GABA双联体模式是一致的，频率间隔类似于13 Hz。2004年出版Wiley-Liss，Inc.
A single-shot, two-echo method for the simultaneous detection of multiple-quantum (MQ)-filtered gamma-aminobutyric acid (GABA) and creatine (Cr) was developed and demonstrated in the human brain in vivo at 3 Tesla. The simultaneously measured Cr singlet served as a navigator for the spectral phase of GABA and any frequency shift during measurements due to drift in the static magnetic field (B-0) or subject movement, as well as an internal concentration reference. In addition, the use of a double-band frequency-selective MO filter for C-3 and C-4 methylene protons of GABA provided a very robust measurement of GABA, with excellent suppression of overlapping metabolites such as Cr and glutathione (GSH) in each single scan. Contamination from overlapping macromolecules was also demonstrated to be negligible with this method. The GABA-to-Cr ratio was 0.09 +/- 0.03 (mean +/- SD, N = 17) and the estimated concentration of GABA in the frontoparietal region of the human brain in vivo was 0.66 +/- 0.19 mumol/g (mean +/- SD, N = 17) with the internal reference method, and 0.69 +/- 0.18 mumol/g (mean +/- SD, N = 17) with the external reference method. The observed pattern of GABA doublet was consistent among all subjects, with a frequency separation of similar to13 Hz. Published 2004 Wiley-Liss, Inc.