Antiviral effects if ribavirin and 6-mercapto-9-tetrahydro-2-furylpurine against dengue viruses in vitro.
Antiviral effects if ribavirin and 6-mercapto-9-tetrahydro-2-furylpurine against dengue viruses in vitro.
复制标题
利巴韦林和 6-巯基-9-四氢-2-呋喃基嘌呤在体外对登革热病毒具有抗病毒作用。
DOI：
10.1016/0166-3542(82)90027-4
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发表时间：
1982
影响因子：
7.6
通讯作者：
Halstead,SB
中科院分区：
文献类型：
--
作者：
Koff,WC;ElmJr,JL;Halstead,SB
体外检测了利巴韦林（1-β-d-呋喃核糖基-1,2,4-三唑-3-甲酰胺）和6-巯基-9-四氢-2-呋喃基嘌呤（6-MPTF）对登革热病毒的抗病毒作用。利巴韦林在远低于细胞毒性水平的浓度下显着减少了 LLC-MK2 细胞中 1-4 型登革热病毒的生长（细胞活力通过台盼蓝染料排除法测定）。在利巴韦林处理的登革热病毒感染的细胞培养物中添加鸟苷完全逆转了该药物的抗病毒作用。相比之下，利巴韦林对人外周血白细胞（PBL）中的登革热病毒复制没有影响。 6-MPTF 是次黄嘌呤鸟嘌呤磷酸核糖基转移酶的特异性抑制剂，在 LLC-MK2 细胞或人 PBL 中均不会显着减少登革热病毒的生长。然而，观察到 6-MPTF 和利巴韦林的协同作用，两种药物的联合治疗显着抑制了人类 PBL 中登革热病毒的复制。成功证明利巴韦林和 6-MPTF 联合治疗可显着抑制单核白细胞中的登革热病毒复制，这表明需要评估这种治疗在体内对抗登革热病毒感染的功效。
The antiviral effects of ribavirin (1-β-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) and 6-mercapto-9-tetrahydro-2-furylpurine (6-MPTF) against dengue viruses were examined in vitro. Ribavirin significantly reduced the growth of dengue virus types 1–4 in LLC-MK2 cells at concentrations well below cytotoxic levels (cell viability was determined by trypan blue dye exclusion). Addition of guanosine to ribavirin-treated dengue virus-infected cell cultures completely reversed the antiviral effect of the drug. In contrast, ribavirin had no effect on dengue virus replication in human peripheral blood leukocytes (PBL). 6-MPTF, a specific inhibitor of hypoxanthine-guanine phosphoribosyltransferase, did not significantly reduce the growth of dengue viruses in either LLC-MK2 cells or human PBL. However, synergistic effects of 6-MPTF and ribavirin were observed, as combined treatment of the two drugs markedly suppressed the replication of dengue viruses in human PBL. The successful demonstration that dengue virus replication in mononuclear leukocytes is markedly suppressed by the combined treatment of ribavirin and 6-MPTF signals a need to evaluate the efficacy of this treatment against dengue virus infections in vivo.