The PELPIII glycoproteins in Solanaceae: stylar expression and transfer into pollen tube walls
The PELPIII glycoproteins in Solanaceae: stylar expression and transfer into pollen tube walls
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DOI：
10.1007/s00497-003-0196-2
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发表时间：
2004-01-01
影响因子：
--
通讯作者：
Mariani, C
中科院分区：
文献类型：
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作者：
de Graaf, BHJ;Knuiman, BA;Mariani, C
烟草第三类雌蕊特异性伸展蛋白（PELPIII）在花柱传递组织（TT）的细胞间基质（IM）中积累。授粉后，110-140 kDa的PELPIII从IM易位到花粉管壁中。PELPIII样序列已在几种茄科植物中发现。这些序列在成熟的非授粉花柱中在RNA和蛋白质水平上表达。在烟草属中，有N. alata、N. x sanderae和N. Sylvestris（有翅组）和N.毛状猪笼草N.结果表明，在耳孢属（Tomentosae组）的成熟花柱中，PELPIII的表达水平与耳孢属（N.烟草。PELPIII基因在所研究的最古老的物种，即N。三角叶属（三角叶组）。为研究PELPIII向烟草花粉管壁转运的种间依赖性，以烟草N.烟草雌蕊与异源种N. rustica，N.在中国的分布Trigonophylla和矮牵牛，其中PELPIII同源物在花柱中不存在。免疫细胞学试验表明，N.烟草PELPIII易位到所有三个种的花粉管壁中。因此，这些种的花粉管壁不形成IM化合物如110-140 kDa PELPIII的屏障，并且PELPIII对花粉管生长没有任何可能的影响不可能是由于PELPIII不能转运通过壁。本文从PELPIII的进化起源、花粉雌蕊的相互作用、花柱TT特异蛋白的功能以及花粉管壁的物理性质等方面讨论了这些发现的重要性。
The class III pistil-specific extensin-like proteins (PELPIII) of Nicotiana tabacum accumulate in the intercellular matrix (IM) of the style transmitting tissue (TT). After pollination, the 110-140 kDa PELPIII is translocated from the IM into the pollen tube walls. PELPIII-like sequences have been found in several solanaceous species. These sequences are expressed in mature non-pollinated styles at both RNA and protein level. Of the genus Nicotiana, the species N. alata, N. x sanderae and N. sylvestris (section Alatae), and N. tomentosiformis and N. otophora (section Tomentosae) showed an expression level of PELPIII homologues similar to that in mature styles of N. tabacum. PELPIII genes were absent in the most ancient species studied, namely N. trigonophylla (section Trigonophyllae). To study the species dependence of the translocation of PELPIII into the pollen tube wall in tobacco, interspecific pollinations on N. tabacum pistils were carried out with pollen from the incongruous species N. rustica, N. trigonophylla and Petunia hybrida, where PELPIII homologues are absent in the style. Immunocytological tests showed that the N. tabacum PELPIII is translocated into the pollen tube walls of all three species. Thus, the pollen tube walls of these species do not form a barrier for IM compounds such as the 110-140 kDa PELPIII and the absence of any possible effect of PELPIII on pollen tube growth cannot be due to failure of PELPIII transport through the wall. The importance of these findings is discussed with respect to the evolutionary origin of PELPIII, the pollen pistil interaction, the function of style TT-specific proteins and the physical properties of pollen tube walls.