A BAC-based contig map of the cynomolgus macaque (Macaca fascicularis) major histocompatibility complex genomic region
A BAC-based contig map of the cynomolgus macaque (Macaca fascicularis) major histocompatibility complex genomic region
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DOI：
10.1016/j.ygeno.2006.11.002
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发表时间：
2007-03-01
期刊：
影响因子：
4.4
通讯作者：
Inoko, Hidetoshi
中科院分区：
文献类型：
--
作者：
Watanabe, Atsushi;Shiina, Takashi;Inoko, Hidetoshi
为了促进食蟹猴作为重要的医学和生物学研究实验动物之一的发展，构建食蟹猴BAC文库进行恒河猴和食蟹猴的基因组比较是必要的。在本文中，我们构建了一个食蟹猴BAC文库和一个地图的MHC（Mafa）的基因组区域的基因组组织和核苷酸相似性之间的比较，人类，黑猩猩，恒河猴。BAC文库由221，184个克隆组成，平均插入片段大小为83 kb，提供了六倍的单倍体基因组覆盖率。共使用114个BAC克隆和54个PCR引物组来构建MFIC区域的4.3-Mb重叠群。从选定的MHC亚区的基因组序列的多样性分析表明，猕猴序列的变化相比，猕猴，人类和黑猩猩序列分别为0.48%，4.15%和4.10%。从这些发现中，我们得出结论，BAC文库和Mafa基因组图谱是基因组分析的有用工具，将有重要的应用比较基因组学和确定区域的后果在医学研究中。(c)2006年由Elsevier Inc.出版
The construction of a cynomolgus macaque (Macaca fascicularis, Mafa) BAC library for genomic comparison between rhesus and cynomolgus macaques is necessary to promote the cynomolgus macaque as one of the important experimental animals for future medical and biological research. In this paper, we constructed a cynomolgus macaque BAC library and a map of the MHC (Mafa) genomic region for comparison of the genomic organization and nucleotide similarities between the human, the chimpanzee, and the rhesus macaque. The BAC library consists of 221,184 clones with an average insert size of 83 kb, providing a sixfold coverage of the haploid genome. A total of 114 BAC clones and 54 PCR primer sets were used to construct a 4.3-Mb contig of the MFIC region. Diversity analysis of genomic sequence from selected subregions of the MHC revealed that the cynomolgus sequence varied compared to rhesus macaque, human, and chimpanzee sequences by 0.48, 4.15, and 4.10%, respectively. From these findings, we conclude that the BAC library and Mafa genomic map are useful tools for genome analysis and will have important applications for comparative genomics and identifying regions of consequence in medical research. (c) 2006 Published by Elsevier Inc.