RAPID EPITHELIAL RESTITUTION OF HUMAN AND RABBIT COLONIC MUCOSA
RAPID EPITHELIAL RESTITUTION OF HUMAN AND RABBIT COLONIC MUCOSA
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DOI：
10.1016/0016-5085(89)90640-9
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发表时间：
1989-09-01
期刊：
影响因子：
29.4
通讯作者：
SCHIESSEL, R
中科院分区：
文献类型：
--
作者：
FEIL, W;LACY, ER;SCHIESSEL, R
快速上皮恢复现在被认为是胃和十二指肠的主要防御机制之一。由于目前没有证据表明人体组织中是否会发生恢复，因此本研究检查了浅表损伤后的人和兔结肠粘膜，并监测了光镜和电子显微镜观察到的粘膜恢复的电位差、碱通量、速度和机制。将兔体内结肠腔暴露于100 mM HCl中5 min或将人体外结肠腔暴露于10 mM HCl中10 min，导致兔76%的上皮表面和人95%的上皮表面的浅表粘膜损伤。坏死的上皮细胞从完整的基底膜上成片脱落，形成保护性的粘液层。在兔中，在损伤后15分钟内和在人中30分钟内出现恢复的形态学证据，因为活的非杯状细胞投射出板状足并以λ的速度在裸露的基底层上迁移。损伤后1小时，兔中61%的粘膜表面仍被损伤，2小时后，人粘膜表面的86%被损伤。在接下来的60分钟内，恢复进展迅速，因此在2小时后，兔中只有10%的表面保持未修复，在3小时后，人中只有19%的表面保持未修复。损伤较深的小区域直到损伤后5 h才修复。粘膜损伤后电位差下降，尽管形态修复，但仍未恢复。快速上皮恢复被认为是胃肠道粘膜的基本防御机制，其明显不一定与胃或十二指肠中酸性环境的存在相关。
Rapid epithelial restitution is now considered one of the primary defense mechanisms of the stomach and duodenum. Because there is currently no evidence as to whether restitution occurs in human tissue, this study examined human and rabbit colonic mucosa after superficial injury and monitored to potential differences, alkaline flux, and speed and mechanisms of mucosal restitution as observed with light and electron microscopy. Luminal exposure of the in vivo rabbit colon to 100 mM HCl for 5 min or the in vitro human colon wo 10 mM HCl for 10 min caused superficial mucosal injury to 76% of the epithelial surface in the rabbit and 95% in the human. The necrotic epithelial cells detached in sheets from the intact basal lamina and formed a protective mucoid layer. Morphologic evidence of restitution occurred within 15 min after injury in the rabbit and 30 min in the human, as viable nongoblet cells projected lamellopodia and migrated over the denuded basal lamina at a speed of .apprx. 2 .mu.m/min. One hour after damage 61% of the mucosal surface was still damaged in the rabbit, and 86% of the human mucosal surface was damaged after 2 h. In the following 60 min restitution progressed rapidly, so that only 10% of the surface remained unrepaired in the rabbit after 2 h and 19% in the human after 3 h. Small areas with deeper injury did not repair until 5 h after damage. The potential difference dropped after mucosal injury and did not recover despite morphologic repair. Rapid epithelial restitution is considered to be a basic defense mechanism of the gastrointestinal mucosa that is obvously not necessarily related to the presence of an acidic environment in the stomach or duodenum.