Heat stability of human placental alkaline phosphatase
Heat stability of human placental alkaline phosphatase
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人胎盘碱性磷酸酶的热稳定性
DOI：
10.1136/jcp.18.3.359
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发表时间：
1965
影响因子：
3.4
通讯作者：
S. Posen
中科院分区：
文献类型：
--
作者：
F. Neale;J. S. Clubb;D. Hotchkis;S. Posen
从人胎盘制备的碱性磷酸酶比任何其他已知的人源碱性磷酸酶显示出更大的耐热失活性。在镁的存在下，该酶可在70°C下加热。30分钟而不丧失活性，而其他人碱性磷酸酶在56°C下加热时丧失其大部分活性。在这段时间里。这种热稳定性见于新鲜制备的酶、乙醇分级分离和冷冻干燥的材料以及已注入胎盘碱性磷酸酶的个体的血清。我们的观察结果的临床意义进行了简要说明。
Alkaline phosphatase prepared from human placentae shows greater resistance to heat inactivation than any other known alkaline phosphatase of human origin. In the presence of magnesium this enzyme may be heated at 70°C. for 30 minutes without loss of activity whereas other human alkaline phosphatases lose most of their activity on being heated at 56°C. for this period of time. This heat stability is seen in freshly prepared enzyme, in alcohol-fractionated and freeze-dried material, and in the sera of individuals into whom placental alkaline phosphatase has been infused. The clinical implications of our observations are briefly indicated.