Dynamics of skeletal muscle energetics during ischemia and reperfusion assessed by in vivo 31P NMR
Dynamics of skeletal muscle energetics during ischemia and reperfusion assessed by in vivo 31P NMR
复制标题
通过体内 31P NMR 评估缺血和再灌注期间骨骼肌能量学的动态
作者：
B. Soussi;J. Idström;A. Bylund‐Fellenius;T. Scherstén
用在体31P核磁共振技术研究了大鼠骨骼肌缺血和再灌注时能量代谢的动态变化。已经确定了止血带缺血2和4小时期间磷酸肌酸、无机磷酸盐、ATP峰和细胞内pH值变化的时间过程，随后进行长达24小时的组织再灌注。此外，在比目鱼肌和胫骨肌的ATP和IMP浓度已被确定的高性能液相色谱分析响应缺血和随后的再灌注。结果表明，在缺血的最初几分钟内，pH值出现初始过冲。还表明，肌肉在缺血2小时后完全恢复，而肌肉细胞的能量状态在缺血4小时后再灌注24小时后没有恢复。然而，比目鱼肌比胫骨肌恢复得更好。结果进行了讨论，在氧的可用性，再灌注损伤，IMP积累和不同的肌肉之间的反应不同的纤维组成。
The dynamics of rat skeletal muscle energy metabolism in response to ischemia and reperfusion have been investigated by in vivo 31P NMR spectroscopy. The time course of changes in the phosphocreatine, inorganic phosphate, ATP peaks and in intracellular pH during 2 and 4 h of tourniquet ischemia followed by up to 24 h of tissue reperfusion have been determined. Furthermore, the ATP and IMP concentrations in the soleus and tibialis muscles have been determined by high performance liquid chromatography analysis in response to ischemia and subsequent reperfusion. The results demonstrate an initial overshoot in the pH during the first minutes of ischemia. It is also shown that the muscles recover completely after 2 h of ischemia whereas the energy state of the muscle cell is not restored after 4 h of ischemia followed by up to 24 h of reperfusion. However, the soleus muscle recovers better than the tibialis. The results are discussed in terms of oxygen availability, reperfusion injury, IMP accumulation and different response between muscles with different fibre composition.
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DOI：
10.1172/jci111289
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Lange,LG;Hartman,M;Sobel,BE
通讯作者：
Sobel,BE
哺乳动物肌肉能量学的模式。
DOI：
10.1242/jeb.115.1.165
发表时间：
1985
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Kushmerick,MJ
通讯作者：
Kushmerick,MJ
影响因子：
15.9
作者：
SABINA, RL;SWAIN, JL;HOLMES, EW
通讯作者：
HOLMES, EW
兔红细胞钠-锂逆向转运蛋白不存在显着的钠-氢交换。
DOI：
10.1152/ajpcell.1985.249.1.c63
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Jennings,ML;Adams-Lackey,M;Cook,KW
通讯作者：
Cook,KW