Expression and roles of fatty acid synthase in hepatocellular carcinoma
Expression and roles of fatty acid synthase in hepatocellular carcinoma
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DOI：
10.3892/or.2014.3484
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发表时间：
2014-12-01
期刊：
影响因子：
4.2
通讯作者：
Geng, Zhimin
中科院分区：
文献类型：
--
作者：
Hao, Qiwei;Li, Tao;Geng, Zhimin
细胞代谢异常与肿瘤的发生发展密切相关。脂肪酸合成酶(FASN)是催化脂肪酸合成的关键分子。越来越多的证据表明，FASN在多种恶性肿瘤中都有高表达，它能促进肿瘤细胞内源性脂肪酸的合成，合成的脂肪酸为肿瘤细胞的增殖提供能量。然而，目前还没有关于FASN在肝细胞癌中的表达和功能的系统研究。本研究的目的是从组织学和细胞水平证实FASN在肝癌细胞中的高表达，并构建FASN shRNA真核表达载体以干扰肝癌细胞株SK-Hep-1中FASN的表达，以探讨FASN在肝癌细胞增殖、凋亡、侵袭和迁移中的作用。在本研究中，我们发现FASN在肝细胞癌组织中的表达高于癌旁组织和正常肝细胞系7702(P<0.05)。
Cell metabolism abnormalities are closely related to tumor occurrence and development. Fatty acid synthase (FASN) is the key molecule for catalyzing fatty acid synthesis. Increasing evidence indicates that FASN is highly expressed in a number of malignant tumors; it can promote the synthesis of endogenous fatty acids in tumor cells and then the synthesized fatty acids provide energy for the proliferation of tumor cells. However, there has been no systematic study focusing on FASN expression and function in hepatocellular carcinoma (HCC). The aim of the present study was to verify the high expression of FASN in HCC cells at the histological and cellular levels, and to construct FASN shRNA eukaryotic expression vector for interfering FASN expression in HCC cell line SK-Hep-1, in an effort to explore the role of FASN in the proliferation, apoptosis, invasion and migration of HCC cells. In the present study, we demonstrated that FASN was highly expressed in HCC tissues compared with tumor-adjacent tissue and normal liver cell line 7702 (P