Analysis in human neonates of defective antibody-dependent cellular cytotoxicity and natural killer cytotoxicity to herpes simplex virus-infected cells.
Analysis in human neonates of defective antibody-dependent cellular cytotoxicity and natural killer cytotoxicity to herpes simplex virus-infected cells.
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对人类新生儿的缺陷性抗体依赖性细胞毒性和自然杀伤细胞对单纯疱疹病毒感染细胞的细胞毒性进行分析。
DOI：
10.1093/infdis/150.1.14
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Gonik,B
中科院分区：
文献类型：
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作者：
Kohl,S;Loo,LS;Gonik,B
在对感染单纯疱疹病毒（HSV）的Chang肝细胞的铬释放试验中，与成人细胞相比，新生儿单核细胞（MCs）具有较低的抗体依赖性细胞毒性（ADCC）。新生儿多形核白细胞（PMNL） ADCC与成人相似。在单细胞琼脂糖偶联实验中，HSV抗体IgG显著增加成人MCs、成人PMNLs和脐带血PMNLs的偶联，但脐带血MCs没有。红细胞抗体结位法检测高亲和力IgG Fc受体（FcR）的表达，结果显示成人脐带血FcR与MC FcR之间存在显著差异。PMNL的FcR表达无差异。在IgG和FcR表达的情况下，人干扰素-a增加了新生儿MC黏附，但对MC ADCC没有影响。缺陷的FcR表达和靶细胞粘附可能部分解释了低新生儿mcadcc。此外，脐带血细胞有溶解性或再循环性以及粘附性缺陷。
Human neonatal mononuclear cells (MCs) had low antibody-dependent cellular cytotoxicity (ADCC) compared with cells from adults in a chromium-release assay against Chang liver cells infected with herpes simplex virus (HSV). Polymorphonuclear leukocyte (PMNL) ADCC of neonates was similar to that of adults. In a single-cell agarose conjugation assay IgG antibody to HSV significantly increased conjugation by adult MCs, adult PMNLs, and cord blood PMNLs but not by cord blood MCs. Expression of the high-affinity IgG Fc receptor (FcR) assayed by erythrocyte-antibody rosetting revealed significant differences between adult MC FcR and cord blood FcR. There was no difference in PMNL FcR expression. Human interferon-a increased neonatal MC adhesion in the presence of IgG and FcR expression, but it had no effect on MC ADCC. Defective FcR expression and target cell adhesion may partly explain low neonatal MC ADCC. In addition, cord blood cells have a lytic or recycle, as well as an adherence, defect.