RNA editing and mitochondrial activity in promastigotes and amastigotes of Leishmania donovani.
RNA editing and mitochondrial activity in promastigotes and amastigotes of Leishmania donovani.
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DOI：
10.1016/j.ijpara.2008.10.015
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发表时间：
2009-05
影响因子：
4
通讯作者：
Maslov DA
中科院分区：
文献类型：
--
作者：
Nebohácová M;Kim CE;Simpson L;Maslov DA
Kinetoplast maxicircle DNA sequence organization was investigated in Leishmania donovani, strain 1S LdBob. Gene arrangement in the coding (conserved) region of the maxicircle is collinear with that of most trypanosomatids, with individual genes showing 80-90% nucleotide identity to Leishmania tarentolae, strain UC. The notable exception was an integration of a full-size minicircle sequence in the ND1 gene coding region found in L. donovani. Editing patterns of the mitochondrial mRNAs investigated also followed L. tarentolae UC patterns, including productive editing of the components of respiratory complexes III, IV and V, and ribosomal protein S12 (RPS12), as well as the lack of productive editing in five out of six pan-edited cryptogenes (ND3, ND8, ND9, G3, G4) found in these species. Several guide RNAs for the editing events were localized in minicircles and maxicircles in the locations that are conserved between the species. Mitochondrial activity, including rates of oxygen consumption, the presence and the levels of respiratory complexes and their individual subunits and the steady-state levels of several mitochondrial-encoded mRNAs were essentially the same in axenically grown amastigotes and in promastigotes of L. donovani. However, some modulation of mitochondrial activity between these developmental stages was suggested by the finding of an amastigote-specific component in Complex IV, a down-regulation of mitochondrial RNA-binding proteins (MRP) [define] and MRP-associated protein (MRP-AP) in amastigotes, and by variations in the levels of RPS12, ND3, ND9, G3 and G4 pre-edited transcripts.
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影响因子：
4.8
作者：
Kar, S;Soong, L;McMahon-Pratt, D
通讯作者：
McMahon-Pratt, D
影响因子：
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1.5
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64.8
作者：
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作者：
Holzer, TR;McMaster, WR;Forney, JD
通讯作者：
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